Application of microsatellite markers to study the genetic structure of stellate sturgeon populations (Acipenser stellatus Pallas, ۱۷۷۱) in the south Caspian Sea

Population genetic structure of stellate sturgeon (Acipenser stellatus) in ۱۹۷ adult specimens from four fishery regions along the Iranian coastline of the southern Caspian Sea was investigated using microsatellite markers. Out of ۱۵ microsatellite primers, ۱۱ loci were produced, in which ۱۰ of them were polymorphic and ۱ was monomorph. Totally, ۱۸۴ alleles were identified and on average ۱۳.۱ alleles per locus were found (ranged ۸ to ۱۸ alleles). All sampled regions contained unique alleles. Average observed and expected heterozygosity was ۰.۶۶۷ and ۰.۸۵۴, respectively and significant genetic differences between ۴ regions were observed (p£۰.۰۱). Deviations from Hardy-Weinberg equilibrium were found in most cases. Population differentiation test was modest and significant (p£۰.۰۱). Based on FST estimate (p£۰.۰۱), more than one population of stellate sturgeon is identified in the south Caspian Sea. Therefore, fishery management for restocking and conservation of gene pool is highly recommended.

Keywords:

Stellate sturgeon , Population genetic , Microsatellite , South Caspian Sea