Genetic Diversity among Economically Important Zataria multiflora Accessions through ISSR Markers: The Main Step for Breeding and Exploitation Programs

Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
View: 196

This Paper With 10 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_JABR-8-4_007

تاریخ نمایه سازی: 15 دی 1400

Abstract:

Introduction: Zataria multiflora is an important medicinal plant of the Lamiaceae family in Central and Southern Iran. This plant is at the risk of extinction as a result of wasteful harvesting due to growing demand and high economic value.Materials and Methods: In this study, the genetic diversity of ۲۵ different accessions of Z. multiflora, collected from provinces including Hormozgan, Fars, Sistan and Balouchestan, Bushehr, Yazd, Kerman, and Isfahan were examined using Inter Simple Sequence Repeat (ISSR) markers. To extract the DNA, five samples taken from the leaves of each accession were transformed into bulks; then, their concentrations were homogenized and ۱۵ primers were used for the remainder of the experiment.Results: The primers produced ۸۳ polymorphic strands altogether, with an average polymorphism percentage of ۷۷.۳۰%; meanwhile, the highest polymorphism percentage (۱۰۰%) was achieved via primers including ۸۱۶D, ۸۲۴H, ۸۳۶P, and ۸۴۴S. The lowest polymorphism percentage was obtained from ۸۱۱C and ۸۳۴N primers. The results obtained from the Jaccard similarity coefficient in the NTYSIS software showed that the genetic similarity of Z. multiflora accessions varies between ۰.۳۲-۰.۸۲. The lowest similarities were observed in accessions taken from Fanuj and Ashar, Mehriz, and NasrAbad, and two accessions of Mehriz and Khafr. However, the highest similarities were seen among accessions of NalShah GhandAab and Kerman. In principal component analysis, three of the first components explained ۴۰.۴۴% of changes in the entire data. Following a cluster analysis based on the UPGMA algorithm, accessions were classified into six groups.Conclusions: It can be concluded that the ISSR markers are suitable for examining the genetic diversity of Z. multiflora accessions.

Authors

Soolmaz Meamari

Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Hormozgan, Bandar Abbas, Iran

Alireza Yavari

Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Hormozgan, Bandar Abbas, Iran

Mahdi Bikdeloo

Department of Horticultural Sciences, Faculty of Agriculture & Natural Resources, Arak University, Arak, Iran

Tahereh Hashemi

Department of Horticultural Sciences, Faculty of Agriculture and Natural Resources, University of Hormozgan, Bandar Abbas, Iran