Genetic Diversity among Economically Important Zataria multiflora Accessions through ISSR Markers: The Main Step for Breeding and Exploitation Programs

Introduction: Zataria multiflora is an important medicinal plant of the Lamiaceae family in Central and Southern Iran. This plant is at the risk of extinction as a result of wasteful harvesting due to growing demand and high economic value.Materials and Methods: In this study, the genetic diversity of ۲۵ different accessions of Z. multiflora, collected from provinces including Hormozgan, Fars, Sistan and Balouchestan, Bushehr, Yazd, Kerman, and Isfahan were examined using Inter Simple Sequence Repeat (ISSR) markers. To extract the DNA, five samples taken from the leaves of each accession were transformed into bulks; then, their concentrations were homogenized and ۱۵ primers were used for the remainder of the experiment.Results: The primers produced ۸۳ polymorphic strands altogether, with an average polymorphism percentage of ۷۷.۳۰%; meanwhile, the highest polymorphism percentage (۱۰۰%) was achieved via primers including ۸۱۶D, ۸۲۴H, ۸۳۶P, and ۸۴۴S. The lowest polymorphism percentage was obtained from ۸۱۱C and ۸۳۴N primers. The results obtained from the Jaccard similarity coefficient in the NTYSIS software showed that the genetic similarity of Z. multiflora accessions varies between ۰.۳۲-۰.۸۲. The lowest similarities were observed in accessions taken from Fanuj and Ashar, Mehriz, and NasrAbad, and two accessions of Mehriz and Khafr. However, the highest similarities were seen among accessions of NalShah GhandAab and Kerman. In principal component analysis, three of the first components explained ۴۰.۴۴% of changes in the entire data. Following a cluster analysis based on the UPGMA algorithm, accessions were classified into six groups.Conclusions: It can be concluded that the ISSR markers are suitable for examining the genetic diversity of Z. multiflora accessions.

Keywords:

Shirazi thyme , population , Domestication , Cluster analysis , PCR , Molecular marker