Effects of conditioned medium of spleen cells treated with morphine on ۴T۱ cancer cell line

Introduction: Although there is some documentation about the effect of morphine on cancer cells or immune cells, there is no information about the function of morphine on the interaction between immune cells and cancer cells. The aim of this study was to investigate the effects of the conditioned medium of morphine-treated spleen cells on ۴T۱ breast adenocarcinoma cancer cells. Methods: The isolated splenocytes from Balb/c mice were cultured in the presence of phytohemagglutinin (۱ mg/ml) and different concentrations of morphine (۰, ۱ /, ۰.۵, and ۱ μM) for ۷۲ hours. The cells were then cultured for ۲۴ hours in the presence of morphine-free medium and FBS and the conditioned medium was harvested. ۴T۱ cells were exposed to the conditioned medium for ۲۴ h. Results: Treatment with morphine reduced the levels of TNF-α, nitric oxide, and myeloperoxidase in the conditioned medium. The viability and proliferative potential of ۴T۱ cells increased significantly concurrent with increasing the expression of p۵۳ and Bcl-۲ genes and decreased expression of caspase-۳ gene after the co-cutler of ۴T۱ cells with the conditioned medium isolated from morphine-treated spleen cells. Conclusion: Due to the reduction in the production of immune mediators by morphine treatment, it is better to use alternative mechanisms to relieve pain in people with cancer as much as possible.

Keywords:

spleen cells , breast cancer , morphine