Expression of estrogenic response genes to different concentration of ۱۷ß-estradiol in male mosquitofish (Gambusia yucatana)

The estrogenic effects of endocrine disrupting compounds in fish are not reversible and can reduce populations. Sensitive methods such as Q-PCR, Western blot, microarrays, have been used to determine changes in gene expression and this predict the effects before they become irreversible. The present study was designed to detect the expression of the estrogen receptors vitellogenin and pregnane X indicates that they are potentially useful molecular markers for detecting the presence of endocrine disrupting compounds in the environment. The results demonstrate that the exposure to the hormone ۱۷ß-estradiol induced the expression of estrogenic response genes in male mosquito fish (G. yucatana) and this is related to the alterations to the endocrine system caused by the hormone.

Keywords:

Gene expression , Biomarkers , Endocrine disrupting compounds (EDCs) , Mosquitofish(Gambusia yucatana) , vitellogenin , Q-PCR.