Prevalence of the Integrons and ESBL Genes in Multidrug-Resistant Strains of Escherichia coli Isolated from Urinary Tract Infections, Ardabil, Iran

Background and Objective: Integrons play an essential role in disseminating drug resistance genes among bacteria. The aim of this study was to determine the prevalence of integrons, and Extended-Spectrum β-Lactamase (ESBL) genes in Escherichia coli (E. coli) isolates collected from patients with urinary tract infection (UTI) referred to teaching hospitals in Ardabil, Iran. Materials and Methods: In this descriptive, cross-sectional study (۲۰۱۷-۲۰۱۸), ۱۶۳ isolates of E.coli were collected from patients with UTI. The drug susceptibility pattern of these isolates to ۱۲ common antibiotics was investigated using the disk diffusion method based on CLSI guidelines. The prevalence of class ۱, ۲ ,۳ integrons and ESBL genes was verified by the PCR method. Finally, the genetic variation of isolates was analyzed using the ERIC-PCR method. Results: Of ۱۶۳ isolates, ۱۳۸ (۸۴.۷%) isolates were multidrug-resistance (MDR) strains. The lowest and highest antibiotic resistance was reported to nitrofurantoin and ampicillin, with a resistance rate of ۱.۲% and ۸۹.۶%, respectively. The incidence of class ۱ and ۲ integrons was obtained in ۳۹.۹% and ۱۴.۱% of the isolates, respectively. Class ۳ integron was not found in any of the isolates. Based on the ERIC-PCR fingerprinting method, ۴ ERIC types were detected. Conclusion: Our study showed that E. coli isolates taken from patients mainly were MDR strain and resistant to many of the common antibiotics used to treat urinary tract infections. Using the correct dose of medication and multidrug therapy would be effective in reducing the incidence of antibiotic resistance.

Keywords:

Escherichia coli , Integron , Multi-drug resistance , UTI , اشریشیا کلی , عفونت ادراری , مقاومت چند دارویی , اینتگرون