Prevalence of the Integrons and ESBL Genes in Multidrug-Resistant Strains of Escherichia coli Isolated from Urinary Tract Infections, Ardabil, Iran

Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_IJMM-16-1_006

تاریخ نمایه سازی: 27 بهمن 1400

Abstract:

Background and Objective: Integrons play an essential role in disseminating drug resistance genes among bacteria. The aim of this study was to determine the prevalence of integrons, and Extended-Spectrum β-Lactamase (ESBL) genes in Escherichia coli (E. coli) isolates collected from patients with urinary tract infection (UTI) referred to teaching hospitals in Ardabil, Iran. Materials and Methods: In this descriptive, cross-sectional study (۲۰۱۷-۲۰۱۸), ۱۶۳ isolates of E.coli were collected from patients with UTI. The drug susceptibility pattern of these isolates to ۱۲ common antibiotics was investigated using the disk diffusion method based on CLSI guidelines. The prevalence of class ۱, ۲ ,۳ integrons and ESBL genes was verified by the PCR method. Finally, the genetic variation of isolates was analyzed using the ERIC-PCR method. Results: Of ۱۶۳ isolates, ۱۳۸ (۸۴.۷%) isolates were multidrug-resistance (MDR) strains. The lowest and highest antibiotic resistance was reported to nitrofurantoin and ampicillin, with a resistance rate of ۱.۲% and ۸۹.۶%, respectively. The incidence of class ۱ and ۲ integrons was obtained in ۳۹.۹% and ۱۴.۱% of the isolates, respectively. Class ۳ integron was not found in any of the isolates. Based on the ERIC-PCR fingerprinting method, ۴ ERIC types were detected. Conclusion: Our study showed that E. coli isolates taken from patients mainly were MDR strain and resistant to many of the common antibiotics used to treat urinary tract infections. Using the correct dose of medication and multidrug therapy would be effective in reducing the incidence of antibiotic resistance.

Authors

Soheyla Barzegar

Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil Iran

Mohsen Arzanlou

Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil Iran

Amir Teimourpour

Department of Epidemiology and Biostatistics, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran

Majid Esmaelizad

Central Labaratory, Razi Vaccine and Serum Research Institute, Karaj, Iran

Mehdi Yousefipour

Department of Infectious and Tropical Diseases, Tehran University of Medical Sciences, Tehran, Iran

Jafar MohammadShahi

Departments of Infectious Diseases, School of Medicine, Ardabil University of Medical Sciences, Ardabil, Iran

Roghayeh Teimourpour

Department of Microbiology, School of Medicine, Ardabil University of Medical Sciences, Ardabil Iran

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