Genetic analysis of the complete G gene of viral hemorrhagic septicemia virus (VHSV) genotype Ie isolates from Turkey
Publish place: Iranian Journal of Fisheries Sciences، Vol: 17، Issue: 1
Publish Year: 1396
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JIFRO-17-1_006
تاریخ نمایه سازی: 27 بهمن 1400
Abstract:
Viral hemorrhagic septicemia virus (VHSV) is an enveloped non-segmented, single-stranded, negative-sense RNA virus that belongs to the Novirhabdovirus genus of the family Rhabdoviridae. This virus causes economically significant diseases in farmed rainbow trout, in Turkey, which is often associated with the transmission of pathogens from European resources. In this study, moribund rainbow trout (Oncorhynchus mykiss) samples were collected during an outbreak of VHSV in a rainbow trout fish farm in Bolu Province of Turkey in ۲۰۰۶. In addition, two VHSV strains were isolated from wild turbot (Scophthalmus maximus) in Trabzon Province of the Black Sea region of Turkey during a field survey. We have sequenced the full-length glycoprotein (G) gene of three VHSV isolates and compared them with ۲۵ previously published gene sequences. Based on a complete gene nucleotide sequence, Turkish VHSV isolates were classified into class Ie of genotype I, which is closely related to GE-۱.۲ isolate (۹۷.۱-۹۷.۵% nucleotide identity and ۹۸.۲-۹۸.۴% amino acid identity) found in Georgia more than ۳۰ years ago. These isolates could be an indigenous type of VHSV distributed in the Black Sea. On the other hand, Turkish isolates have ۹۷.۵-۹۷.۶% nucleotide identity and ۹۸.۸-۹۹% amino acid identity with Finnish, Danish, and Norwegian isolates which are classified under Ib and Id. These results suggest that Turkish VHSV isolates may have orginated from Europe and co-circulated with indigenous strains which can threaten the aquaculture industry in Turkey.
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Authors
H. Albayrak
Department of Virology, Faculty of Veterinary Medicine, Ondokuz Mayis University, Samsun, Turkey
H. Isidan
Department of Virology, Faculty of Veterinary Medicine, Cumhuriyet University, Sivas, Turkey
G. Kalayci
Department of Virology, Bornova Veterinary Control Institute, İzmir, Turkey
E. Ozan
Department of Virology, Veterinary Control Institute, Samsun, Turkey
V.N. Vakharia
Institute of Marine and Environmental Technology, University of Maryland Baltimore County, Baltimore, MD, ۲۱۲۰۲, USA
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