Occurrence and histopathology of Ascocotyle tenuicollis metacercaria in gill of platyfish (Xiphophorus maculatus) imported to Iran

The objective of this study was to determine the prevalence of metacercarial infection in plathyfish imported to Iran. A total of ۱۰۰ imported live common platyfish (Xiphophorus maculatus) from approximately ۲۴ shipments were collected from a quarantine facility in Iran in ۲۰۰۷ and examined in our veterinary laboratory. Among ۸۰۰ examined gill arches about ۱۳۰۰ cysts were found in different locations of gill filaments and were identified as a heterophyid parasite metacercaria Ascocotyle (Ascocotyle) tenuicollis  Price, ۱۹۳۵. The prevalence of  this parasite was ۵۸%, the maximum and minimum quantity of metacercarial cysts per infected platyfish were ۷۰ and ۱۰, respectively. The adult worms are non-pathogenic and are intestinal parasites of piscivorous birds (e.g. Heron and Egret) and mammals. The first intermediate hosts are snails and the second intermediate hosts are centrarchiid, cyprinodontid, mugilid, and poeciliid  fishes. Histopathologic examination of the infected fish gills revealed cartilage proliferation around metacercarial cysts, hyperplasia, hypertrophy and fusion in the affected gill filaments. To our knowledge, this is the first report of infection with Ascocotyle tenuicollis  in the imported platyfish in Iran.

Keywords:

Metacecaria , Platyfish , Gill , Pathology , Ascocotyle , Iran