Identification of bester hybrids (female Huso huso Linnaeus, ۱۷۵۸ and male sterlet Acipenser ruthenus Linnaeus, ۱۷۵۸) using AFLP molecular technique
Publish place: Iranian Journal of Fisheries Sciences، Vol: 11، Issue: 2
Publish Year: 1391
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JIFRO-11-2_016
تاریخ نمایه سازی: 21 اسفند 1400
Abstract:
In this study Amplified Fragment Length
Polymorphism (AFLP) was applied to species identification of bester hybrids.
Hybrids identification was performed by comparison of electrophoresis profiles
with parental species. The simultaneous occurrence of diagnostic bands fixed in
the parental species, genetic distance and identification and cluster analyses
(UPGMA) allow a correct identification. We used ۸ primer combinations (Eco+۳,
Mse+۴) and a total of ۲۵۰ bands (size range ۴۰-۱۰۰۰ bp) were generated.
Primer combinations of (E-AAT, M-CGAT) and (E-AAG, M-CGAT)
produced diagnostic bands in hybrids and parental species. Moreover, the
results of genetic identification showed that Bester hybrids are more similar
to beluga (Huso huso) (۰.۶۸) in comparison with sterlet (Acipenser
ruthenus) (۰.۴۵). The results suggested
that this technique could be suitable for precise identification of species and
inter-generic hybrids like bester.
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