Investigation of the synergist effect extract of licorice and Elaeagnus Angustifolia on the chondrogenesis of human adipose-derived stem cells on fibrin scaffold

OBJECTIVES In today's investigation, the use of mesenchymal stem cells in the reconstructive medical profession and scientific research has been considered and also has made significant progress. Herbal medicines have recently taken a special place in medical treatment and research. The study was performed to evaluate the effect of Elaeagnus Angustifolia extract and licorice extract on cartilage repair using ADSC in a fibrin scaffold medium. METHODS In the study, stem cells were isolated from adipose tissue by mechanical and enzymatic digestion of subcutaneous adipose tissue of healthy humans. In a DMEM-enriched medium, ۱% penicillin and streptomycin were cultured in ۲۵ cm۳ flasks and incubated at ۳۷ ° C and ۵% CO۲ pressure. After the third passage, the cells are implanted in fibrin scaffolds. Cells cultured in scaffolding are divided into four groups: ۱- control group ۲- licorice extract (۲۵μg/۱), ۳- Elaeagnus Angustifolia extract (۱۰μg/۱), and ۴ - licorice extract + Elaeagnus Angustifolia extract. After ۱۴ days, cell viability was assessed using the trypan blue test and the expression of collagen genes type ۲, SOX۹, Agrrican and type ۱۰ was measured using the RT- PCR method. The data were analyzed using one-way ANOVA followed by the Tukey test in SPSS software.RESULTSExamination of chondrocyte-specific genes in different groups showed that the resulting fat stem cells were in the process of chondrogenesis during ۱۴ days. Gene expression in different groups was compared with the control group. Examination of collagen and Aggrecan and SOX۹ gene expression, in general, showed that in the specified time, the presence of Elaeagnus Angustifolia and licorice extract was effective on gene expression (P-value ≤۰.۰۵). significantly higher in the group that received licorice and elm at the same time.CONCLUSIONS: This study confirms that licorice extract and fruit extract in the presence of fibrin scaffolding could be an effective method in improving cartilage lesions.

Keywords:

licorice , Elaeagnus Angustifolia , chondrogenesis , human adipose-derived stem cells , fibrin , scaffold