Prevalence of Tetracycline Resistance Genes tet (A, B, C, ۳۹) in Klebsiella pneumoniae Isolated from Tehran, Iran
Publish place: Iranian Journal of Medical Microbiology، Vol: 16، Issue: 2
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IJMM-16-2_006
تاریخ نمایه سازی: 28 فروردین 1401
Abstract:
Background and Objective: Klebsiella pneumoniae is one of the three pathogens that has become a global disease control and treatment problem due to its resistance to common antibiotics. For this reason, it is crucial to study the genes that cause antibiotic resistance in it. Therefore, the aim of this study was to investigate the phenotypic and genotypic frequency of tetracycline resistance in clinical isolates of K. pneumoniae in Tehran, Iran.
Materials and Methods: In this study, ۱۰۰ isolates of K. pneumoniae isolated from clinical samples (urine) during ۲۰۱۸-۲۰۱۹ were studied. In addition to microbial and biochemical phenotypic tests, genotypic tests were conducted to determine the frequency of antibiotic resistance genes tet (A, B, C, ۳۹).
Results: Out of ۱۰۰ isolates of K. pneumoniae, ۴۹ isolates were resistant to tetracyclines. The results of multiplex PCR showed that ۳۱ samples were positive for tetA gene, ۸ isolates for tetB gene, ۲۱ samples for tetC and, and ۸ isolates for tet۳۹. None of the isolates were positive for all four tetracycline genes.
Conclusion: The results of this study showed that the isolates were positive for at least one gene and at most ۲ tetracycline resistance genes. The tetA gene showed the highest frequency and the lowest frequency was demonstrated by tetB. The highest binary combination of genes was tetA-tetC, and the lowest was tetA-tet۳۹.
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Authors
Zeinab Ahmadi
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Zahra Noormohammadi
Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran
Reza Ranjbar
Molecular Biology Research Center, Systems Biology and Poisonings Institute, Baqiyatallah University of Medical Sciences, Tehran, Iran
Payam Behzadi
Department of Microbiology, College of Basic Sciences, Shahr-e-Qods Branch, Islamic Azad University, Tehran, Iran
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