The influence of hydro alcoholic extract of Satureja mutica zinc Oxide nanoparticle and zinc complex against coagulase gene expression on standard and clinical isolated of methicillin resistant staphylococcus aureus

Methicillin-resistant Staphylococcus aureus (MRSA) is one of the most important hospital pathogens that cause of severe morbidity and mortality worldwide. Coagulase gene (Coa) is a pathogenicity factor of this bacterium which found in all pathogenic strains. The aim of this study was to evaluate the antibacterial effect of hydroalcoholic extract of Satureja mutica, zinc oxide nanoparticle and zinc complex alone and simultaneously on inhibiting the growth of clinical and standard of coagulase gene expression isolates of MRSA. Minimum Inhibitory Concentration (MIC), cell viability and zone of inhibition were determained by microdilution method. MTT assay and disk diffusion method were performed against MRSA strains that possess coa gene respectively. In addition, the changes in expression of this gene were investigated by RT-PCR in MIC concentration. Hydroalcoholic extract of Satureja mutica, zinc oxide nanoparticle, and copper nano-complex showed antibacterial and inhibitory effects on MRSA growth. Additionally, a significant inhibitory effect was seen on coagulase gene expression in presence of hydroalcoholic extract of Satureja mutica. Frurthermore, the MIC for clinical and standard MRSA were determined as ۱۵۰۰ and ۳۰۰۰ µg/ml for the Satureja mutica extract, ۲۰ and ۴۰ µg/ml for the zinc oxide nanoparticle, and ۲۰ and ۴۰ µg/ml for the Zinc complex respectively. After performing RT-PCR, the results of electrophoresis showed that Satureja mutica extract in MIC induced an inhibitory effect on coagulase gene expression. Using Satureja mutica extract with nanoparticles in MIC concentration showed a synergistic effect in coa gene expression. However, no effect was observed on housekeeping arcC gene. Zinc oxide nanoparticles also have inhibitory effects on the growth of bacteria but no inhibitory effect was observed on expression coa gene.

Keywords:

MRSA , CuO , Cu complex , coagulase gen , Satureja Mutica