Evaluation of the effect of polyethyleneimine-based nanoparticles containing scorpion venom (Mesobuthus eupeus) on the growth of Sf9 cell line

Despite the benefits of chemical toxins in killing pests, they are dangerous to organisms and the environment. Examining the mechanism of scorpion peptides, it can be said that scorpion venom is a factor in pest control. In this study, we tried to determine the effect of polyethyleneimine-based nanoparticles in increasing the efficiency of the lethal effect of scorpion venom Mesobuthus eupeus on the Sf9 cell line. Sf9 cells were placed in plate wells 96. Then concentrations of 10, 25, and 35 μg/ml scorpion venom and polyethyleneimine-based nanoparticles were added to the Sf9 cell. The cells were exposed to toxins for 24 hours. Then results were determined using the MTT test. This study showed that with increasing the concentration of PEI derivatives, toxicity in the Sf9 cell line decreases; also considering the effect of PEI hydrophobicity modification on increasing PEI transmission efficiency (LMW), the ability to transfer M. eupeus scorpion venom at a concentration of 10 μg/ml to Sf9 cell line with minimal toxicity increases. All PEI derivatives were able to effectively deliver the scorpion venom M. eupeus peptide.

Evaluation of the effect of polyethyleneimine-based nanoparticles containing scorpion venom (Mesobuthus eupeus) on the growth of Sf9 cell line Keywords:

Mesobuthus eupeus , scorpion venom , polyethyleneimine , nanoparticles , Sf9 cell line.