Molecular inspection of contamination with Salmonella Enteritidis in Tabriz city aviaries
Publish place: Journal of Zoonotic Disease، Vol: 6، Issue: 1
Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_JZD-6-1_005
تاریخ نمایه سازی: 16 خرداد 1401
Abstract:
The aim of this study was to investigate the occurrence of Salmonella Enteritidis infection between the birds in Tabriz city aviary by molecular method. In this study, the presence of paratyphoid infection in the pet bird market of Tabriz city was investigated for the first time. One hundred six fecal samples were obtained from aviaries in Tabriz urban area. The samples were then transferred to tetrathionate broth culture media. After ۲۴ hours of incubation, samples were transferred to MacConkey agar plates. Samples showing no fermentation of lactose and containing transparent colonies were transferred to salmonella shigella agar plates. In the next stage, the resulting dark colonies, which were determined to be salmonella bacteria, were entered to a multiplex polymerase chain reaction test. Findings showed that ۷ (۶.۶%) out of ۱۰۶ samples were contaminated with salmonella bacteria. In addition, results of multiplex PCR confirmed Salmonella Enteritidis as the source of Salmonella Enteritidis contamination in ۲ (۱.۸۸%) samples. This finding shows that a slight degree of contamination with enteritidis infection is present in birds in Tabriz city aviaries. It can be concluded that there is a slight rate of infection by Salmonella Enteritidis in Tabriz city aviaries. Our findings show that this contamination is latent, and necessary measures should be taken to confront it.
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Authors
Amirreza Tavassoli
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Mohammad Sadegh Madadi
Department of Clinical Sciences, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran
Sahar Nouri Gharajalar
Department of Pathobiology, Faculty of Veterinary Medicine, University of Tabriz, Tabriz, Iran.
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