Correlation of -۴۷۵ IL-۲ Promoter Gene Polymorphisms and the Levels of Serum IL-۲ on the Risk of Multiple Sclerosis

Background: The aim of present study is to asset the IL-۲ promoter gene (SNP -۴۷۵) as a candidate gene for multiple sclerosis (MS) susceptibility. Methods: This study included ۷۰ patients with relapsing – remitting multiple sclerosis (RRMS) and ۵۰ healthy controls. Following the extraction of genomic DNA from peripheral blood, frequency of genotypes and alleles of SNP -۴۷۵ was calculated using Restriction fragment length polymorphism-polymer chain reaction (RFLP-PCR) and then the results were analyzed statistically. Results: The results revealed the unusual ratio for the heterozygous (AT) was ۱.۶۹۷۲ indicating that heterozygous patients were at higher risk of multiple sclerosis than wild homozygous (AA), and homomutant (TT). The results show protective role for - ۴۷۵ IL-۲ promoter among individuals with multiple sclerosis, (O.R: ۰.۴۸۷۲; C.I. ۹۵%: ۰.۱۶۱۷- ۱.۴۶۸۰) and (O.R: ۰.۹۲۷۵; C.I. ۹۵%: ۰.۲۴۷۶ - ۳.۴۷۴۵) for both AA and TT genotypes, respectively. Conclusions: Our results showed that in this population of Iraqi patients, the AT genotype / A allele of -۴۷۵ IL-۲ promoter gene SNP may include attributed factors for MS predisposition.

Keywords:

IL-۲ , Multiple sclerosis , PCR-RFLP SNP.