Synthesis, labeling, formulation and quality control of ۹۹mTc-Tricine-HYNIC-Tyr³-Octreotide, a peptide radiopharmaceutical for imaging Somatostatin receptor positive tumors [Persian]

Somatostatin analoges labeled with different radionuclides are able used for imaging and treatment of somatostatin receptor positive tumors. In this study Tyr³-Octreotide protected at lysine as an analoge of somatostatin was conjugated with bifunctional chelating agent ۶-BOC-hydrazinopyridine-۳-carboxylic acid (BOC-HYNIC) and after deprotecting of conjugate, purification was performed with preparative-RP-HPLC. The conjugated peptide was labeled with ۹۹mTc and tricine as coligand. Radiolabelling efficiency, radiochemical purity, stability in human serum, internalization of receptor bound ۹۹mTc-labeled peptide and biodistribution in normal mice were studied.

Keywords:

Somatostatin , Tyr-Octreotide , HYNIC , Tricine , ۹۹mTc