Production of ۹۹mTc-Antimony Sulfide Colloid for lymphoscintigraphy [Persian]

Introduction: The lymphatic system provides one of the main paths for the spread (Metastasis) of cancer from one part of body to another. Hodgkin’s diseases, lymphocytic leukemia, various metastatic disease and many lymph node disorders can be assessed by lymphoscintigraphy. Radionuclide lymphoscintigraphy has been used for many years to define lymphatic drainage of melanoma. The most common radiopharmaceuticals used for lymphoscintigraphy are ۹۹mTc-SC, ۹۹mTc-antimony sulfide colloid and ۹۹mTc-HSA-nanocolloid. Preparation of ۹۹mTc-antimony sulfide colloid has been chosen among other colloids. Materials and Methods: For antimony colloid preparation, hydrogen sulfide gas was passed through D.W. until saturation. Antimony potassium tartrate is then added to the solution to form Sb۲S۳ colloid. The colloid was stabilized with P.V.P. Excess H۲S was removed by bubbling with nitrogen. The preparation was then filtered through a ۰.۲۲μm membrane filter and aliquots containing ۱.۰۱۷mg Sb۲S۳ were dispensed into kit vials. Labeling was accomplished by adding ۹۹mTcO-۴ and HCl to the vial then heating it at ۱۰۰°C in boiling water bath for ۱۰ min. The pH was adjusted by adding a phosphate buffer. Results: The radiochemical purity of ۹۹mTc-antimony trisulfide colloid by ITLC-SG/normal saline was more than ۹۵ percent. The amount of Sb in reaction vial was ۰.۷۲۹mg. Conclusion: The study demonstrated that our formulation of antimony sulfide, which has ۰.۰۴۸۶mg (Sb) in ۰.۲ml for injection per patient (Total volume after labeling with ۹۹mTc is ۳ml).

Keywords:

Lymphoscintigraphy , ۹۹mTc-antimony trisulfide colloid