Balancing The Expression of K-ras and CD۸۲ Genes By Magnetic Nano-oleuropein as a New Mechanism for Inhibition of AGS Gastric Cancer Cells

۱) Background: Gastric cancer is the fourth most common cancer and the second leading cause of cancer-associated fatality in the world. During the recent decade, nanoparticles have been used widely to reduce the severity and also the treatment of cancers. The current study was performed to transfer Oleuropein into AGS cancer cells using paramagnetic nano-particles and to evaluate their effects on the cancer cell line. ۲)Methods:  In this research, nine concentrations of magnetic nano-oleuropein (۰, ۰.۱۵, ... ۳۳۳.۳۳ and ۱۰۰۰ µg/mL ) were applied against AGS cells in in-vitro condition with three replicates in the form of a completely randomized statistical design (CRD), and cell viability was investigated using MTT and Flow Cytometry assays. To determine the molecular mechanism of this effect, the relative expression of K-ras and cd۸۲ genes were investigated using the Real-time PCR assay. ۳)Results: Our results for inhibition of cancer cells by different concentrations of magnetic nano-oleuropein showed that the inhibition rate of AGS cancer cells was dependent on the concentration and exposure time of the drug. The relative expression of K-ras oncogene decreased at the concentrations higher than ۴.۱۲ μg/mL and increased at lower concentrations (p-value <۰.۰۱). Also, the expression of cd۸۲ gene at the concentrations below IC۵۰ (۲۳.۶ μg/mL) increased while at higher concentrations reduced significantly. ۴)Conclusion: Balancing the expression of K-ras and cd۸۲ oncogenes through the p۵۳ protein could be one of the molecular reasons for inhibiting the AGS cells by magnetic nano-oleuropein. Therefore, magnetic nano-oleuropein at IC۵۰ concentration plays a key role in inhibiting gastric cancer cells by the creation of an expression balance between K-ras and cd۸۲ genes.

Keywords:

Nano-oleuropein , K-ras , cd۸۲ , Gastric Cancer , Real-time PCR , Flow Cytometry