Analysis of DRD۱ gene expression pattern in patients with glaucoma

Background: Glaucoma is a term used to describe a group of diseases that progressively weaken the optic nerve and cause vision loss and eventually blindness. Glaucoma is one of the most common and controversial eye diseases. Dopamine, a neurotransmitter in the eye, regulates ciliary blood flow and aqueous humour production, which affects intraocular pressure and glaucoma. Based on previous studies on dopamine receptor ligands, it was concluded that the effect of dopamine on fluid production is stimulated by dopamine receptor ۱ (D۱) activation. Expression and translation of the DRD۱ gene cause the production of the D۱ receptor. Based on this, it was hypothesized that changes in DRD۱ gene expression may play a role in the development of glaucoma. The aim of this study was to analyze DRD۱ gene expression in patients with glaucoma. Materials and Methods: In this study, a statistical population of ۴۰ people including ۲۰ patients and ۲۰ healthy individuals was used to analyze the effect of DRD۱ gene expression on patients with glaucoma. After RNA extraction and cDNA synthesis, the Real-time quantitative PCR (qRT-PCR) technique was used and its data were analyzed by SPSS software and Mann-Whitney statistical method. Results: The results of this study indicate a significant difference (P = ۰.۰۱۵) in the expression of the DRD۱ gene in patients with glaucoma compared to the control group.

Keywords:

Glaucoma , dopamine receptor D۱A , gene expression , Receptors , Dopamine , Real-Time Polymerase Chain Reaction