Gas Chromatography-Mass Spectrometry Analysis and Inhibitory Activity Against Candida albicans ATCC ۱۰۲۳۱ of the Leave Ethanolic Extract From Citrus aurantifolia (Christm.) Swingle
Publish place: Avicenna Journal of Medical Biochemistry، Vol: 9، Issue: 2
Publish Year: 1400
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_MEBIO-9-2_002
تاریخ نمایه سازی: 30 مرداد 1401
Abstract:
Background: Candida albicans is one of the important infectious yeasts that is associated with candidiasis,
including oral candidiasis. The extracts of various herbal materials are potential for treating candidiasis.
Objectives: The objectives of this study were to determine phytochemical constituents of the leaf ethanolic
extract of Citrus aurantifolia with gas chromatography-mass spectrometry (GC-MS) and to investigate the
inhibiting effect of this extract on the planktonic growth of C. albicans.
Methods: The fresh leaves of C. aurantifolia were macerated overnight with ethanol. The extract was
analysed with GC-MS. C. albicans ATCC ۱۰۲۳۱ was used in this study. The well-diffusion procedure
was applied to detect the anti-candida activity qualitatively. Finally, real-time planktonic growth was
employed for detecting the anti-candida activity quantitatively.
Results: GC-MS analysis revealed four dominant components in the ethanolic leaf extract of C. aurantifolia,
namely, limonene, geraniol, phytol, and caryophyllene. The extract inhibited the growth of C. albicans
either under the agar diffusion test or real-time planktonic growth. The specific growth rate of C. albicans
was slower in the liquid culture with the extract. The specific growth rates of the ۰ (control), ۱۳.۳, and
۲۶.۶ μg/mL were ۰.۵۸۲, ۰.۳۸۴, and ۰.۲۷۲, respectively. Eventually, the yields of the treated growth with
۰ (control), ۱۳.۳, and ۲۶.۶ μg/mL were OD۸۵۰ of ۴.۵, ۳.۰, and ۳.۷, respectively.
Conclusion: The leaf ethanolic extract of C. aurantifolia contains bioactive compounds which have anticandida
activity. Thus, it is a good material for new anti-candida ingredients in the future.
Keywords:
Authors
Monica Pospassari
Faculty of Medicine and Health Sciences, Krida Wacana Christian University, Jakarta, Indonesia
Gloria Stefanie
Faculty of Medicine and Health Sciences, Krida Wacana Christian University, Jakarta, Indonesia
Kris Herawan Timotius
Faculty of Medicine and Health Sciences, Krida Wacana Christian University, Jakarta, Indonesia- Research Center for Jamu and Herbal Medicine, Jakarta, Indonesia