Anti-inflammatory and Antioxidant Properties of PLGA Nanoparticles Produced From Kombucha Extract on A۲۷۸۰ Human Ovarian Cancer Cell Line

Background: Anti-cancer agents encapsulated in nanoparticles (NPs) can result in higher efficiency. Kombucha is a fermented tea beverage, and previous reports support its anti-cancer properties. Objectives: The present study aimed to evaluate the anti-cancer and anti-inflammatory properties of poly (lactic-co-glycolic acid) loaded Kombucha NPs (PLGA-K-NPs) against the A۲۷۸۰ human ovarian cancer cell line. Methods: The antioxidant activity was analyzed using ferric reducing ability of plasma and ۲,۲’-azinobis( ۳-ethylbenzothiazoline-۶-sulfonic acid) assays, along with the measurement of catalase (CAT) gene expression. The gene expression of three interleukins (IL-۱β, IL-۶, and IL-۱۰) was also determined to demonstrate the anti-inflammatory properties of NPs. Results: The results revealed the antioxidant effects of PLGA-K-NPs on the studied cell lines by increasing Fe۳+ reduction, inhibiting the free radical formation (P < ۰.۰۰۱), and increasing the expression of the CAT gene (P < ۰.۰۰۱). In addition, NPs could significantly elevate the gene expression of IL-۱۰ (P < ۰.۰۱) as an anti-inflammatory cytokine at a ۴۰ μg/mL concentration, while reducing the expression of IL-۱β and IL-۶, and inflammatory cytokines at all tested concentrations (P < ۰.۰۱). Conclusion: According to the obtained results, PLGA-K-NPs have anti-inflammatory and anti-oxidant properties, therefore, they can be considered as a compound in the treatment of ovarian cancer. However, it needs to be further investigated in animal studies to clarify more details.

Keywords:

Antioxidant effects , Inflammation , Kombucha extract , Ovarian cancer , Poly (Lactic-co-glycolic acid) nanoparticles