Phenotypic and genotypic characterization of a marine bacterium isolated from Sundarbans, Bangladesh

The mangrove ecosystem is a largely unexplored source for bacteria with potentiality to produce biologically active secondary metabolites. Consequently, we set our research study to isolate, characterize and screen a bacterium producing bioactive compounds from marine soil of largest mangrove forest of the world, Sundarbans, Bangladesh. A total number of ۳۹ marine bacterial colonies were isolated and purified. Representative bioactive isolate, ANAM-۵ was characterized using phenotypic and genotypic procedures since it exhihibited highest antibacterial activity against a series of test organisms. The ۱۶S rDNA sequence of the strain ANAM-۵ showed close similarity with only one bacterium Planctomyces brasiliensis, complete genome (۹۹.۶۴%). But the cultural, morphological, physiological and biochemical characteristics of the strain and P. brasiliensis were completely different. So, it may not be logical to assign the strain ANAM-۵ to the genus Planctomyces. Thus, to confirm the taxonomic position of the strain and to have a correct solution from this taxonomic problem, further studies is needed in respect to the DNA relatedness studies, fatty acid profiling, DNA-DNA hybridization, matrix-assisted laser desorption/ionization time-of- flight (MALDI-TOF), metabolic finger profiling using BIOLOG, ribotyping, small subunit (SSU) sequences, cell wall composition and other characterizations.

Keywords:

Phenotypic , Genotypic , Marine bacteria , Sundarbans