Cross talk of vasopressin conditioned cell therapy in ischemic heart disease: Role of oxidative stress markers

Objective(s): Background: Impaired coronary blood flow causes cardiac ischemia. Cellular therapy is a new approach to the treatment of myocardial ischemia. This study aimed to investigate the effect of adipose tissue-derived mesenchymal stem cells (AD-MSCs) conditioned with vasopressin on oxidative stress, perivascular collagen, and angiogenesis caused by myocardial infarction (MI) in rats. Materials and Methods: We divided ۴۰ male albino Wistar rats into ۴ groups; Control group; No intervention; in experimental groups, after it generated induced MI on models, it divided into three groups: Vehicle group (۱۵۰ μl of cell-free culture medium received); ASC-MI group (۶× ۱۰۶ AD-MSC received) and AVP-ASC-MI group (received ۶ × ۱۰۶ AD-MSC conditioned with ۱۰ nM vasopressin). Then, histologic parameters and anti-oxidant enzymes were evaluated ۷ days post-MI cell injection. Results: Arterial muscle diameter improved and collagen deposition around the coronary arteries decreased in cell-received groups compared with the vehicle group. Malondialdehyde (MDA), catalase (CAT), (GSH) Glutathione, and Total Anti-oxidant Capacity (TAC) parameters were not significantly different between the cells received groups compared with the vehicle group. But the Catalase (CAT) parameter in the ASC-MI group had a significant increase from the control group. Conclusion: We prepared direct evidence that intramyocardial injection of AD-MSCs reveals the positive cardiac remodeling post-MI in rats, and these useful effects can be more enhanced by administrating injection of conditioned ADSCs with vasopressin.

Keywords:

Anti-Oxidants , Catalase , Coronary vessels , Glutathione , Malondialdehyde , Mesenchymal stem cells , Myocardial infarction , Myocardial schemia Oxidative stress , Vasopressins