Silver nanoparticles modulate high fat high carbohydrate diet induced metabolic changes in rats via reducing lipid peroxidation, PDGF-β and insulin resistance

Objective(s): Non-alcoholic fatty liver disease (NAFLD) is strongly linked with insulin resistance and type-۲ diabetes through various metabolic changes. The current study was designed to investigate the modulatory effects of silver nanoparticles (AgNPs) on metabolic and inflammatory changes activated during NAFLD. Materials and Methods: Three doses of AgNPs (۱۰۰, ۱۵۰, and ۲۰۰ μg/kg/day for ۴ weeks) were tested in a high-fat high carbohydrate diet (HFHCD) induced NAFLD model in rats. Results: Sinificant (P<۰.۰۵) improvement in dyslipidemia, hyperglycemia, and insulin levels by AgNPs was observed and more notably in the group that received ۲۰۰ μg/kg/day AgNPs. Acute phase inflammatory protein C-reactive protein and monocytes chemoattractant protein-۱ were significantly (P<۰.۰۵) lowered by AgNPs. Inline, lipid peroxidation and PDGF-β levels were significantly (P<۰.۰۵) reduced in groups that received different doses of AgNPs. Furthermore, AgNPs especially in the large dose (۲۰۰ μg/kg/day) significantly decreased (P<۰.۰۵) the measured level of the inflammatory cytokines (IL-۱β, IL-۶, and TNF-α) compared with the HFHCD group level.Conclusion: Collectively, results propose the ability of AgNPs to modulate metabolic changes accompa-nying NAFLD through reducing lipid peroxidation and targeting inflammatory cytokines mediating insulin resistance.

Keywords:

Fatty liver , insulin resistance , Nanoparticles , Silver