Impact of caffeic acid on the testicular damages in D-galactose induced aging model in mice

Objective(s): Aging is a biological phenomenon that causes various disorders and diseases in body systems such as the reproductive system. One of the important factors in aging is oxidative stress, which facilitates the aging process through various mechanisms. The aim of this study is the investigation of  effects of caffeic acid on the testicular damages in D‑galactose induced aging model in mice. Materials and Methods: Forty male mice were randomly divided into ۵ groups (n=۸): ۱) Control, ۲) Sham, ۳) Aging, ۴) Aging + caffeic acid, and ۵) Caffeic acid. Aging was induced through daily injection of D-Galactose (۳۰۰ mg/kg, intraperitoneal) for ۶ weeks. Caffeic acid (۶۰ mg/kg, intraperitoneal) was injected daily for ۶ weeks. One day after the last injection mice were killed and the testicle and epididymis were removed. Then, sperm parameters, factors of oxidative stress, and histopathological changes were evaluated. Results: The results showed that aging significantly decreased the count, motility, and viability of sperm, and increased abnormal sperm and sperm DNA fragmentation in contrast to the control group (P<۰.۰۵). In addition, MDA levels increased significantly in this group, and SOD, GPx, and TAC activity decreased (P<۰.۰۵). Histological studies also showed the destruction of seminiferous tubules, and Johnson’s score decreased (P<۰.۰۵). Caffeic acid  administration significantly improved the above disarrays (P<۰.۰۵). Conclusion: The results showed that caffeic acid reduces the adverse effects of aging on spermatogenesis in mice by reducing oxidative stress and increasing antioxidant defenses.

Keywords:

Aging , Caffeic acid , D-galactose , Oxidative stress , Spermatogenesis