Effects of crocin on T-bet/GATA-۳ ratio, and miR-۱۴۶a and miR-۱۰۶a expression levels in lung tissue of ovalbumin-sensitized mice

Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
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JR_IJBMS-25-10_015

تاریخ نمایه سازی: 11 مهر 1401

Abstract:

Objective(s): Although various studies have revealed the beneficial effects of crocin (derived from saffron), such as anti-inflammatory, anti-cancer, antioxidant, and immune modulator, however, its exact mechanism is unknown. The present study aimed to investigate the effect of crocin on the expression ratio of T-bet/GATA-۳ as an indicator of altered immune responses in the lung tissue of ovalbumin (OVA)-sensitized mice. In addition, the effect of crocin on the expression level of miR-۱۴۶a and miR-۱۰۶a in the lung tissue OVA-sensitized mice was investigated.Materials and Methods: Mice were randomly divided into five groups (n=۶): Control; OVA, OVA + Crocin ۲۵, OVA + Cro ۵۰, and OVA + Cro۱۰۰ groups. Crocin was administrated intraperitoneally at doses of ۲۵, ۵۰, and ۱۰۰ mg/kg for five consecutive days. One day after asthma induction, animals were euthanized, and lungs were sampled for pathological and gene expression analysis.Results: OVA-sensitization led to increased inflammation and histopathological changes in the lung tissue of mice. In addition, GATA-۳ expression increased (P<۰.۰۰۱) and T-bet expression decreased (P<۰.۰۰۱) in OVA-sensitized groups. The T-bet/GATA۳ ratio was also reduced markedly in asthma groups (P<۰.۰۰۱). Furthermore, increased expression of miR-۱۴۶a and miR-۱۰۶a levels was evident in the lung tissue of OVA-sensitized mice (P<۰.۰۰۱ for both). Intervention with high concentrations of crocin (۵۰ and ۱۰۰ mg/kg) significantly reduced airway inflammation, GATA-۳ expression, miR-۱۴۶a expression, and miR-۱۰۶a expression and corrected the T-bet/GATA-۳ ratio (P<۰.۰۵ to P<۰.۰۰۱).Conclusion: Treatment with crocin led to a decrease in the severity of lung inflammation in OVA-sensitized mice, which is probably through the reduction of the T-bet/GATA-۳ ratio, and mir-۱۴۶a and mir-۱۰۶a expression level.

Authors

Mohammad Reza Aslani

Lung Diseases Research Center, Ardabil University of Medical Sciences, Ardabil, Iran

Zahra Jafari

Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran

Reza Rahbarghazi

Drug Applied Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

jafar Rezaie

Solid Tumor Research Center, Research Institute for Cellular and Molecular Medicine, Urmia University of Medical Sciences, Urmia, Iran

Aref Delkhosh

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

Mahdi Ahmadi

Stem Cell Research Center, Tabriz University of Medical Sciences, Tabriz, Iran

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  • Pelaia G, Renda T, Gallelli L, Vatrella A, Busceti MT, ...
  • Krug N, Hohlfeld JM, Kirsten AM, Kornmann O, Beeh KM, ...
  • Vale-Pereira S, Todo-Bom A, Geraldes L, Schmidt-Weber C, Akdis CA, ...
  • Kim SH, Hong JH, Lee YC. Oleanolic acid suppresses ovalbumin-induced ...
  • Yuan Y, Yang B, Ye Z, Zhang M, Yang X, ...
  • Kim SH, Hong JH, Yang WK, Kim HJ, An HJ, ...
  • Ahmadi M, Rahbarghazi R, Aslani MR, Shahbazfar AA, Kazemi M, ...
  • Guo HW, Yun CX, Hou GH, Du J, Huang X, ...
  • Ahmadi M, Rahbarghazi R, Shahbazfar A-A, Keyhanmanesh R. Monitoring IL-۱۳ ...
  • Rahbarghazi R, Kihanmanesh R, Rezaie J, Mirershadi F, Heiran H, ...
  • Collison A, Herbert C, Siegle JS, Mattes J, Foster PS, ...
  • Akhavanakbari G, Babapour B, Alipour MR, Keyhanmanesh R, Ahmadi M, ...
  • Fang C, Lu W, Li C, Peng X, Wang Y, ...
  • Panganiban RP, Wang Y, Howrylak J, Chinchilli VM, Craig TJ, ...
  • Ghasemi Z, Rezaee R, Aslani MR, Boskabady MH. Anti-inflammatory, anti-oxidant, ...
  • Saadat S, Aslani MR, Ghorani V, Keyhanmanesh R, Boskabady MH. ...
  • Khazdair MR, Saadat S, Aslani MR, Shakeri F, Boskabady MH. ...
  • Boskabady MH., Aslani MR., Mansuri F., Amery S. Relaxant effect ...
  • Boskabady MH, Aslani MR. Relaxant effect of Crocus sativus (saffron) ...
  • Aslani MR, Amani M, Masrori N, Boskabady MH, Ebrahimi HA, ...
  • Ghobadi H, Abdollahi N, Madani H, Aslani MR. Effect of ...
  • Rahimi G, Shams S, Aslani MR. Effects of crocin supplementation ...
  • Nam KN, Park YM, Jung HJ, Lee JY, Min BD, ...
  • Yarijani ZM, Pourmotabbed A, Pourmotabbed T, Najafi H. Crocin has ...
  • Ahmadi M, Rahbarghazi R, Soltani S, Aslani MR, Keyhanmanesh R. ...
  • Taghizadeh S, Keyhanmanesh R, Rahbarghazi R, Rezaie J, Delkhosh A, ...
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  • Lloyd CM, Hessel EM. Functions of T cells in asthma: ...
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  • Korani S, Korani M, Sathyapalan T, Sahebkar A. Therapeutic effects ...
  • Zeinali M, Zirak MR, Rezaee SA, Karimi G, Hosseinzadeh H. ...
  • Du Q, Gu X, Cai J, Huang M, Su M. ...
  • Barrett NA, Austen KF. Innate cells and T helper ۲ ...
  • Abdi H, Aganj Z, Hosseinzadeh H, Mosaffa F. Crocin restores ...
  • Poursamimi J, Shariati-Sarabi Z, Tavakkol-Afshari J, Mohammadi M. A significant ...
  • Sharma A, Kumar M, Ahmad T, Mabalirajan U, Aich J, ...
  • Feng MJ, Shi F, Qiu C, Peng WK. MicroRNA-۱۸۱a, -۱۴۶a ...
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  • Weidner J, Bartel S, Kılıç A, Zissler UM, Renz H, ...
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  • Saki N, Abroun S, Soleimani M, Mortazavi Y, Kaviani S, ...
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