Association of ART۳ rs۶۸۳۶۷۰۳ polymorphism with non-obstructive Azospermia in Iranian men

Backgrounds: if a couple does not have children within one year after unprotected sexualactivity, they suffer from infertility, which affects both men and women. Various factors affectmale infertility, about ۱۵ to ۳۰% of which are genetic abnormalities. It is also predicted that mostunknown cases of infertility are based on a genetic defect. Studies on the ART۳ gene haveshown that it is associated with Non-obstructive azoospermia (NOA). Therefore, in this study,we investigated the association of ART۳ rs۶۸۳۶۷۰۳ polymorphism with NOA in the Iranianinfertile male population.Materials and Methods: In this study, ۱۰۰ genomic DNA samples of NOA individuals and ۱۰۰samples of control (fertile) individuals were received from Royan Research Institute, and thenusing Tetra ARMS-PCR technique, rs۶۸۳۶۷۰۳۳ polymorphisms were examined in both groups.Results: The results showed that the frequency of mutant A allele in the patient group washigher than the control group, but in both groups, the frequency of the normal G allele washigher than the mutant allele and no significant relationship was observed between the patientand healthy groups (P -۰.۰۶۷ and OR-۰.۶۷۲). Also, the findings of genotype showed that AAgenotype is ۱۶% in patients and ۷% in the control group and normal GG genotype is ۴۵% inpatients and ۵۳% in the control group, which indicates the higher frequency of mutated genotypein patients and the frequency of normal genotypes is higher in healthy individuals.Conclusion: The study of rs۶۸۳۶۷۰۳ polymorphic relationship in Iranian male population didnot show a significant relationship with NOA.

Keywords:

Infertility , Non-obstructive Azospermia , ART۳ gene , polymorphism rs rs۶۸۳۶۷۰۳