Association between DROSHA rs۶۴۲۳۲۱ polymorphism and breast cancer in Iranian women

Backgrounds: Breast cancer is the most common cancer for women worldwide and is mainfactor of cancer mortality. The incidence rates of breast cancer in the developing countries areincreasing. Single nucleotide polymorphisms (SNPs) in microRNA biosynthesis pathways mayresult in the less or gain of microRNA function that can act as an oncogenic or tumorsuppressive.Dysregulation of microRNA biogenesis pathway genes is involved in the initiationand progression of several human cancers including breast cancer. SNPs in microRNAbiogenesis pathway genes are related to breast cancer risk. One of the most important genes inthis field is DROSHA. Previous studies have shown that rs۶۴۲۳۲۱ is related remarkably todifferent cancer risks.Materials and Methods: We performed a case-control examination of ۱۰۰ breast cancer casesand ۱۰۰ healthy controls to evaluate the association between rs۶۴۲۳۲۱ in DROSHA and breastcancer risk. Genomic DNA was extracted from blood samples. ARMS PCR was performed fordetection of the rs۶۴۲۳۲۱ by designed primers.Results: Statistical analysis showed that rs۶۴۲۳۲۱ polymorphism located in ۳’ untranslatedregion (UTR) of DROSHA gene was related to the enhanced risk of breast cancer.Conclusion: Altogether, our results indicated that presence of DROSHA rs۶۴۲۳۲۱polymorphism could be proper tumor markers in patients with breast cancer.

Keywords:

Breast cancer , DROSHA , rs۶۴۲۳۲۱ , ARMS PCR , SNP