Effect of alterations in miR-۴۸۳-۳p expression on the expression of SMAD۴ gene in breast cancer patients

Backgrounds: SMAD۴ is a down-stream effector of transforming growth factor-beta (TGF-B)signaling pathway. Although the tumor suppressor role of this prognostic biomarker has beenpreviously studied in many malignancies, its role as a prognostic biomarker in breast cancer hasyet to be confirmed. The regulatory effect of microRNA-۴۸۳-۳p (miR-۴۸۳-۳p) as a tumorsuppressor on SMAD۴ has also been investigated, and a correlation has been reported in somemalignancies such as the pancreatic cancer. This study aimed to assess the expression of miR-۴۸۳-۳p and its effect on the expression of SMAD۴ gene in breast cancer patients.Materials and Methods: The expression of SMAD۴ gene was evaluated in ۵۱ paired tumoraltissue/adjacent healthy tissue specimens by real-time polymerase chain reaction (PCR). GAPDHgene was used as the control (reference) gene. The correlation of SMAD۴ expression withclinicopathological features was also evaluated. The expression of miR-۴۸۳-۳p was thenevaluated in ۱۶ SMAD۴ negative tumoral specimens by real-time PCR, to assess its effect on theexpression of SMAD۴ by REST ۲۰۰۹ software. Data were analyzed by the Chi-square test.Results: The results showed no significant correlation between the expression of miR-۴۸۳-۳pand SMAD۴ (P > ۰.۰۵).Conclusion: It appears that alterations in the expression of miR-۴۸۳-۳p have no significanteffect on the expression of SMAD۴ gene in breast cancer patients.

Keywords:

Breast neoplasms , SMAD۴ , Gene expression , miR-۴۸۳-۳p , Real-time Polymerase Chain Reaction