Geopora cooperi, a new hypogeous ascomycete record for Iran mycobiota

The Pezizales are an order of the subphylum Pezizomycotina within the phylum Ascomycota. Members of this order are characterized by unitunicate asci that typically open by rupturing to form a terminal or eccentric lid or operculum (Hansen & Pfister ۲۰۰۶). Of the ۱۶۳۸ Pezizales species known so far, Geopora Harkn. (Pyronemataceae) is represented by ۲۳ species (Kirk et al. ۲۰۰۸). Geopora spp. are characterized by entirely or partially hypogeous, globular, semi-globular or cup-shaped ascocarps, whitish, greyish or yellowish grey hymenium, cylindrical, ۸-spored and operculate asci, generally bifurcate, septate and hyaline paraphyses, ellipsoid, smooth ascospores mostly with one or two larger oil drops and some smaller oil drops (Tamm et al. ۲۰۱۰, Perić & Perić ۲۰۱۱). The members of the Geopora spp. are widely distributed in the Northern Hemisphere. Many researchers have reported Geopora species in North America, central, north, south and southeast Europe, and the Middle East (Tamm et al. ۲۰۱۰, Ashraf & Khalid ۲۰۱۲, Guo et al. ۲۰۱۹, Saba et al. ۲۰۱۹, Uzun & Kaya ۲۰۱۹). Saber & Pegler reportedthe presence of G. arenicola (Lév.) Kers in Iran as Sepultaria arenicola (Ershad ۲۰۰۹). In this study, two specimens of G. cooperi associated with Pinus nigra were collected in Khorassan Razavi province (Iran) in ۲۰۲۰ (Fig. ۱a) with the following characteristics: Ascomata ۵۰–۵۵ mm in diameter, globose, subglobose to oblong, irregularly convoluted, peridium dark brown and covered with light brown hairs, gleba white to pinkish-white, highly convoluted, convolutions close but separate (Fig. ۱b, c). Hymenium ۲۰۰–۲۵۰ μm thick. Asci ۱۸۰–۲۷۰(۲۱۵) × ۱۵–۲۲(۱۶) μm, cylindrical, ۸-spored, non-amyloid and tapering at the base (Fig. ۱d, e). Paraphyses cylindrical, septate, swollen at the tips, nearly of the same level as ascus, enlarged at the apex up to ۱۱.۵ μm (Fig. ۱f). Ascospores ۱۸–۲۷(۲۳) × ۱۳–۱۷(۱۴) μm, smooth, hyaline, broadly elliptical, thin-walled, generally with a large central guttule (Fig. ۱g, h). Ectal excipulum ۱۲۰ μm, ۴–۵ cells thick, dark brown, textura globulosa,cells oblong to circular in section, smooth, walls thick and dark brown, ۲۴–۴۰ × ۱۸–۲۷ μm, cells on the outside darker than the inner cells (Fig. ۱i). Excipular hairs hyphae-like, branched, very long to ۱۰۰۰ μm, ۵–۱۷ μm wide, blunt at the tips and dark walls (Fig. ۱j). All these characteristics were consistent with other authors’ descriptions for G. cooperi (Tamm et al. ۲۰۱۰, Guo et al. ۲۰۱۹).While Geopora cooperi isconsidered as a very rare species by Perić & Perić (۲۰۱۱), itwas widely reported from Europe (Austria, Sweden, Norway, Denmark, Switzerland, Spain, and Italy) by Nannfeldt (۱۹۴۶), Moser (۱۹۶۳), Burdsall (۱۹۶۸), Soleilhac (۱۹۷۲), Ortega et al. (۱۹۸۱), Moreno et al. (۱۹۹۱), and Montecchi & Dal Forno (۱۹۹۵). This species is hereby reported new to the mycobiota of Iran. The mycorrhizal association of Geopora species with various conifers and deciduous trees such as Pinus ponderosa Lawson & Lawson (Fujimura et al. ۲۰۰۵), Pinus edulis Engelm., Cercocarpus ledifolius Nutt., Salix linearistipularis (Franch.) K. S. Hao, Quercus garryana Douglas ex Hook. (Frank et al. ۲۰۰۹), Quercus douglasii Hook., and Arn. (Smith et al. ۲۰۰۶), Picea abies (L.) Karst.(Tedersoo et al. ۲۰۰۶), Epipactis atrorubens (Hoffm.) Besser. (Shefferson et al. ۲۰۰۸), Abies grandis (Dougl.) Lindl., Cedrus spp., and Populus spp. (Tamm et al. ۲۰۱۰) have been confirmed by molecular techniques. Fujimura et al. (۲۰۰۵) have reported G. cooperi form mycorrhiza with Pinus ponderosa. Tedersoo et al. (۲۰۰۶) have identified ectomycorrhizal Geopora spp. on root tips both in coniferous and deciduous woodlands. They also have found Geopora spp. occurred in various early- and late-successional woodlands in acidic to alkaline forest soils, in addition to cinder and burnt soils. Association of G. cooperi with P. nigra, has been previously reported by Burdsall (۱۹۶۸) from the USA. Specimen examined: Iran: Khorassan Razavi province, under Pinus nigra, solitary, on ground, ۲۴ July ۲۰۲۰ (IRAN ۱۶۶۹۶F).  

Keywords:

Ascomycete , new record , Iran , Pinus