Introduction to NGS platforms and their role in plant genetic modification

DNA sequencing is one of the most important techniques in the field of molecular biology, according to which the order of nucleotides can be determined in a piece of DNA. There are several different methods for DNA sequencing. Today, with advances in the field Nanotechnology and bioinformatics, methods have developed to increase the speed and efficiency of DNA sequencing, which has made it possible to carry out large projects such as sequencing the human genome cheaper, more accurately and more quickly. Today, DNA sequencing is an integral part of modern science. This technique is used in various fields of microbiology, including tracking infectious diseases and studying the diversity of microbial communities. There are different ways to sequence DNA. But each of these techniques has its advantages and disadvantages. To get acquainted with these methods, This article is a review that analyzes the generations of sequencing from the past to the present that has been collected from articles, reputable book sites and articles.

Keywords:

Maxam-Gilbert , Pyrosequencing , illumina , Helicos Biosiences