Methanol Extract of Basella Alba Leaf Enhances Glucose Utilization in Nicotine Treated Male Wistar Rats

Background: Nicotine has been reported to exert adverse effects on insulin sensitivity, predispose individuals to metabolic syndrome, and induce decreased functionality of the pancreas. The present study evaluated the influence of methanol extract of Basella Alba leaf on glucose utilization in nicotine treated male Wistar rats.Methods: Twenty male rats, weighing ۲۰۰-۲۴۰g were divided into four groups of five animals each as follows; Healthy Control (H-C) rats, which were given no treatment but placebo, Nicotine Control (N-C), which received ۱.۰ mg/kg of nicotine, Low Dose Nicotine + Basella Alba group (LDN-Ba), which received ۰.۵ mg/kg nicotine and ۲۰۰mg/kg of MeBa, and High Dose Nicotine + Basella Alba group (HDN-Ba) that were given ۱.۰ mg/kg of nicotine and ۲۰۰ mg/kg of MeBa.Results: Following acute nicotine exposure, FBG levels were significantly higher (p ˂ ۰.۰۵) in NC, LDN-B and HDN-B rats when compared to H-C. Likewise, OGTT showed a significant (p ˂ ۰.۰۵) derangement in N-C and HDN-B when compared to HC and LDN-B groups. Body weight, weight and relative weight of pancreas were significantly decreased (p ˂ ۰.۰۵) in all nicotine treated groups when compared to the healthy control group. Histopathology also revealed general distortion of pancreatic histoarchitecture in the nicotine control rats. A significant decrease (p ˂ ۰.۰۵) in the blood glucose level and improved OGTT was observed in LDN-B rats after four weeks treatment with Basella Alba compared with N-C.Conclusion: The findings revealed that the dysfunction in glucose metabolism caused by nicotine toxicity is dose dependent and the administration of methanol extract of Basella Alba leaf ameliorate these effects to a greater extent in low dose than in high dose nicotine group.

Keywords:

Methanol extract , Basella Alba , Glucose Utilization , Nicotine