Determination of Astaxanthin in Trout by High Performance Liquid Chromatography Coupled with Visible Detection

Background: Astaxanthin is widely and naturally distributed in marine animals including, crustaceans, such as shrimp, crabs, and fish, such as salmon and trout. Methods: A total of ۲۰ trout samples were collected from local market and were analyzed for astaxanthin via HPLC-UV. Analyte extraction was done by water, methanol, dichloromethane, and acetonitrile ۴.۵:۸۵:۵:۴۵.۵, v/v/v/v. Results: Despite the optimal conditions, analytical property of the method containing lower limit of quantitation (۲.۰۰ ng g-۱) and upper limit of quantitation (۱۰۰۰ ng g-۱), linearity (r۲=۰.۹۹۲), and recovery (۱۰۳%) were acceptable. The results showed the range of ۱۰۵ ± ۰.۰۹ - ۸۷۵ ± ۰.۰۲۰ ng g-۱. Conclusion: The method was successfully used to quantify the target analytes in all trout samples.

Keywords:

Astaxanthin , High performance liquid chromatography , Fish , Upper limit of quantification (ULOQ) , lower limit of quantification (LLOQ)