Contamination control in Iranian seedless barberry micropropagation

Iranian seedless barberry is a species with high degree of contamination in in vitro culture. The current study was aimed to investigate the effects of mercuric chloride (HgCl۲), sodium hypochlorite (NaOCl) + HgCl۲, HgCl۲ + ۸-hydroxyquinoline, cefotaxime + streptomycin, HgCl۲ + shoot type, HgCl۲ + explant position and HgCl۲ + shoot color on the reduction of contamination. The results showed that the combined use of NaOCl and HgCl۲ was more effective than their single use, but the contamination rate was relatively high. Interestingly, the results showed that explants sterilized with ۱۰۰ mg/L HgCl۲ and cultured in the medium containing ۱۵۰ mg/L ۸-hydroxyquinoline exhibited the lowest fungal contamination (۱۵%), while those treated with ۵۰ mg/L HgCl۲ and cultured in the ۸-hydroxyquinoline-free medium showed the highest fungal contamination (۹۶%). Furthermore, treatment of ۷۵ mg/Lcefotaxime + ۷۰ mg/L streptomycin exhibited no bacterial contamination compared to the control with ۵۸% bacterial contamination. Explants prepared from suckers and treated with ۱۵۰ mg/L HgCl۲ showed the lowest rates of fungal (۱۶%) and bacterial (۱۳%) contamination. The results also showed that explants collected from upper position had the lowest rates of fungal (۲۰.۸۷%) and bacterial (۱۸.۴%) infection. Explants taken from pinkish-colored shoots showed surprisingly the lowest rates of fungal contamination (۱۳.۳۳%), bacterial contamination (۱۰%) and bud mortality (۳۶.۴۷%). In general, choice of explants from upper position of pinkish-colored suckers, sterilization of them with ۱۰۰ mg/L HgCl۲ for ۵ min and their culture in medium containing ۱۵۰ mg/L ۸-hydroxyquinoline can be recommended for reducing the contamination.

Keywords:

۸-hydroxyquinoline , Bud , Contamination , Iranian seedless barberry , Mercury , Micropropagation