Anti-RAGE (Receptor Advanced Glycation End products) Antibody Improves Diabetic Retinopathy in Rats via Hypoglycemic and Anti-inflammatory Mechanism
Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_RBMB-11-3_004
تاریخ نمایه سازی: 18 دی 1401
Abstract:
Background: Receptor advanced glycation end products (RAGE) activation plays an essential role in diabetic retinopathy (DR) progression. This study was aimed to explore the role of anti-RAGE antibodies (RAGE antagonists) in inhibiting DR progression through their hypoglycemic and anti inflammatory mechanism in diabetic retinopathy induced rats.
Methods: A total of ۳۰ male Wistar rats were randomly divided into five group. The group was consisted of normal control group, DR group without treatment, DR group with anti-RAGE ۱ g/kg BW, ۱۰ g/kg BW, and ۱۰۰ g/kg BW. To assess the diabetic retinopathy, fundus photographs were taken every week using a camera with ۱۶x magnification placed in front of the rat's eyes. Blood glucose was checked by the glucose oxidase-peroxidase method. Retinal TNF- levels and VEGF were examined using an enzyme-linked immunosorbent assay (ELISA) kit.
Results: The finding of this study showed that anti-RAGE treatment at dose of ۱۰ and ۱۰۰ g/kg BW, HbA۱c levels were significantly higher (p< ۰.۰۵) compared to the normal control group but significantly lower (p< ۰.۰۵) than in the diabetes group. The mean blood vessel diameter in the DR+anti-RAGE ۱۰ and ۱۰۰ g/kg BW groups was significantly lower than in the diabetic retinopathy group (p< ۰.۰۵). The administration of anti-RAGE ۱۰ and ۱۰۰ g/kg BW showed the ability to significantly reduce VEGF levels compared to the DR group (p< ۰.۰۵).
Conclusions:This study revealed at doses of ۱۰ and ۱۰۰ g/kg BW, anti-RAGE antibodies improved diabetic retinopathy in Wistar rats through hypoglycemic effects and anti-inflammatory mechanisms.
Keywords:
Anti-RAGE (Receptor Advanced Glycation End products) , Diabetic Retinopathy , Glycated Hemoglobin A , Hypoglycemic Agents , Peroxidases , Vascular Endothelial Growth Factor A
Authors
Ramzi Amin
Department of Ophthalmology, Faculty of Medicine Universitas Sriwijaya/ Dr Moh Hoesin General Hospital, Palembang, Indonesia
Tiara Bunga Indiarsih
Specialized Residency Training, Faculty of Medicine Universitas Sriwijaya/Dr Moh Hoesin General Hospital, Palembang, Indonesia.
Prima Maya Sari
Department of Ophthalmology, Faculty of Medicine Universitas Sriwijaya/ Dr Moh Hoesin General Hospital, Palembang, Indonesia.
Petty Purwanita
Department of Ophthalmology, Faculty of Medicine Universitas Sriwijaya/ Dr Moh Hoesin General Hospital, Palembang, Indonesia.
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