The Effect of Endurance Activity on Expression of sox۶ Gene of Left Ventricle in Male Wistar Rat

Background & Aims: The expression of myocardium gene can be affected by endurance activity; besides, sox۶ transcription factor influences its formation. This study aimed to investigate the effect of ۱۴ weeks of endurance activity on expression of sox۶ gene of left ventricle in male Wistar rats. Methods: Forteen rats were housed under the controlled conditions and after adjusting with endurance program, were randomly assigned into two groups of control and experimental. For the experimental group, an endurance program (۱۴ weeks, ۶ days/week, ۶۰ minutes/day, and velocity of ۳۰ m/minutes) on motorized treadmill was performed. Then, the rats were anesthetized and sacrificed ۴۸ hours after the end of the last session and the left ventricle was removed. Real-time polymerase chain reaction (RT-PCR) method was used to determine the expression levels of sox۶ gene in the left ventricle. The collected data were evaluated using t-test. Results: The ratio of left ventricle weight (LVW) to the body surface area (BSA) was significantly (P=۰.۰۱۰) higher in experimental group (۰.۱۶۸ ± ۰.۰۰۸ g/m۲ ) compared to the control group (۰.۱۵۳±۰.۰۰۶g/m۲ ). The ratio of heart weight (HW) to the body surface area was significantly (P = ۰.۰۰۲) higher in experimental group (۰.۲۷۰ ± ۰.۰۱۴) compared to the control group (۰.۲۴۰ ± ۰.۰۱۹), too. In addition, the mean expression value of sox۶ gene of left ventricle was significantly higher in experimental group (P = ۰.۰۰۱), too. Conclusion: It seems that physical activity improves heart functional indices (the ratio of left ventricle and heart weight to body surface area), especial in left ventricle, via increasing the expression ofsox۶ gene.

Keywords:

sox۶ transcription factor , physical activity , Left ventricle