The Frequency of Carbapenemase Genes in Citrobacter Frundii and Citrobacter Koseri Isolated from Clinical Specimens in Imam Reza Hospital, Kermanshah, Iran

Publish Year: 1394
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:

JR_JKMU-22-6_004

تاریخ نمایه سازی: 19 دی 1401

Abstract:

Background & Aims: The inappropriate use of antibiotics has led to antibiotic resistance in microorganisms of Enterobacteriaceae family, especially in carbapenems. The aim of this study was to identify the carbapenemase producing Citrobacter frundii and Citrobacter koseri isolated from clinical specimens. Methods: One hundred Citrobacter isolates from various patient samples in Imam Reza Hospital, Kermanshah, Iran, were identified using the microbiologic differential tests and API-E۲۰ Kit. After antibiotic susceptibility testing with disc, the isolates resisted to carbapenems were screened using MHT (Modified Hodge Test) for the presence of carbapenemases. Then, carbapenemase genes coded Verona integrinencoded metallo-beta-lactamase (VIM), Klebsiella pneumoniae carbapenemase (KPC), Imipenemase (IMP), New Delhi metallo-beta-lactamase-۱ (NDM) were tested using specific primers via polymerase chain reaction (PCR) method. Results: From ۱۰۰ isolates, ۱۱ cases were carbapenem resistant. In the phenotypic screening test, ۲ isolates were positive. PCR on isolates resistant to carbapenams confirmed VIM gene in ۵ isolates: ۳ (۳.۹%) of Citrobacter frundii and ۲ (۱۵.۴%) of Citrobacter koseri. But the genes of blaKPC, blaVIM, blaIMP and blaNDM were not found in isolates. The highest and lowest antibiotic susceptibility were for meropenem (۹۳%) and cefazolin (۱%), respectively. Conclusion: It seems that the prevalence of carbapenemase genes in Citrobacter kosari and Citrobacter frundii was low in Kermanshah; however, VIM gene in these two species is probably more prevalent. This may suggest that most genes have not been high prevalent in this area yet. But, there may be other genes for resistance to carbapenems in our area which need further investigations. The results indicate that carbapenems are still effective antibiotics against Citrobacter species

Authors

Alisha Akya

Associate Professor, Department of Medical Microbiology, Nosocomial Infection Research Center, Kermanshah University of Medical Sciences, Kermanshah, Iran

Somayeh Jafari

MSc Student, Department of Microbiology, Imam Reaza Hospital, Kermanshah University of Medical Sciences, Kermanshah, Iran

Kamal Ahmadi

MSc Student, Department of Medical Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

Azam Elahi

MSc Student, Department of Medical Microbiology, School of Medicine, Kermanshah University of Medical Sciences, Kermanshah, Iran

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