Correlation of Helicobacter pylori vac A s, m Region Genotypes with Different Gastrodoudenal Diseasesin East Azerbaijan Patients

Background& Aims: Gastric adenocarcinoma has been considered as an infectious disease since ۱۹۹۴, when the Helicobacter pylori (H. pylori) infection categorized as a definite class I human carcinogen. H. pylori uses extensive numbers of virulence factors to overcome host defence mechanisms. One independent H. pylori factor that plays an important role in determining H.pylori pathogenesisis vacuolating cytotoxin A(vacA).The purpose of this study was to investigate the frequency of vacA Genotypes, and also its association with disease outcome in infected patients. Methods: Antral gastric biopsy materials were collected from ۸۸ patients with different gastroduodenal diseases. Genotyping of the VacA alleles were determined by PCR methods. Results: Of ۶۴ H.pylori positive patients, ۳۸ were classified as gastritis, ۱۱ as peptic ulcer, and ۱۵ as gastric adenocarcinoma. Four Vac A genotypes were observed, including ۲۰ (۲۲.۷%) for s۱/m۲ (with frequency of relatively equal in all groups), ۱۲ (۱۳.۶%) for s۱m۱ (with the highest frequency in gastric adenocarcinoma and peptic ulcer), ۷ (۷.۹۵%) for s۲m۲ (with the highest frequency in gastritis( and ۳ (۳.۴%) for s۲m۱ (with the highest frequency in peptic ulcer). Conclusion: In this study, no significant relationship was observed between the different genotypes of the vacA and the clinical outcome. Gastric adenocarcinoma also showed significant association with age and gender.

Keywords:

East Azerbaijan , Iran , Helicobacter pylori (H. pylori) , Vacuolating cytotoxin A (vacA) genotypes , Gastric adenocarcinoma , peptic ulcer