Gold Nanoparticles/Cysteic Acid Modified Electrode for Simultaneous Electrochemical Determination of Tramadol and Paracetamol

Electrochemical deposition of gold nanoparticles (AuNPs) and l-Cysteine on glassy carbon electrode was carried out to prepare a modified electrode. The fabricated sensor showed good sensitivity and selectivity for simultaneous determination of paracetamol (PAR) and tramadol (TRA) by square wave voltammetry. Linear ranges of ۰.۱۰ - ۱۰.۷ μM (PAR) and ۰.۵۰ - ۶۳.۶ μM (TRA) were obtained with detection limits of ۰.۰۳ and ۰.۱۷ μM, respectively. The relative standard deviation (RSD %) for simultaneous determinations of PAR (۱۰ µM) and TRA (۳۰ µM) was calculated to be ۳.۱۱ and ۳.۳۰%, respectively (۵ replicate determinations). Moreover, when ۳ different electrodes were used, RSD% for PAR and TRA was ۳.۹۲ and ۴.۰۲%, respectively. The proposed electrode was used successfully in the simultaneous determination of the drugs in spiked human plasma samples.The calculated recoveries for PAR and TRA in blood serum were found to be in the rage ۹۹.۵-۱۰۲ %, with RSD% ۰.۷۱ – ۱.۹%.

Keywords:

Paracetamol , Tramadol , Modified electrode , Cysteic acid , Gold Nanoparticles , Square wave