Molecular and Phenotypic Characterization of Methicillin-Resistant Staphylococcus aureus in Community and Hospital Acquired Infections in Bandar Abbas
Publish place: Infection Epidemiology and Microbiology، Vol: 8، Issue: 3
Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
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شناسه ملی سند علمی:
JR_IEM-8-3_006
تاریخ نمایه سازی: 17 بهمن 1401
Abstract:
Backgrounds: This study aimed to assess the molecular characteristics of methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from community-acquired (CA) and hospital-acquired (HA) infections in Bandar Abbas, southern Iran.
Materials & Methods: This descriptive cross-sectional study was conducted on ۱۱۰ S. aureus strains isolated from ۵۹ outpatients and ۵۱ inpatients during ۲۰۱۸-۲۰۱۹. Antimicrobial susceptibility testing was performed using disc diffusion method. Epsilometer test was used to measure vancomycin minimum inhibitory concentration (MIC). Cefoxitin disc (۳۰ μg) was used to screen MRSA isolates. The presence of mecA gene was examined by PCR method. Staphylococcal cassette chromosome mec (SCCmec) types were detected in S. aureus isolates using multiplex-PCR. Chi-square and Fisher's exact tests were used to analyze the results.
Findings: Out of ۱۱۰ isolates, ۴۵ (۴۰.۹%) isolates carried the mecA gene: ۲۰ (۳۹.۲%) isolates from inpatients and ۲۵ (۴۲.۴%) isolates from outpatients. MRSA isolates showed the highest resistance to azithromycin (۶۹.۸%), tetracycline (۶۰.۴%), and clindamycin (۳۲.۱%), respectively. Vancomycin MIC against MRSA isolates ranged from ۰.۷۵ to ۵ μg/mL. SCCmec type I, III, IV, and V were detected in ۲۰ (۴۴.۴%), three (۶.۷%), ۱۶ (۳۵.۵%), and six (۱۳.۳%) isolates, respectively.
Conclusion: The predominant SCCmec types were type I and type IV, which were detected in CA- and HA-MRSA isolates, respectively. No significant difference in the presence of SCCmec type III and antibiotic resistance was found between CA- and HA-MRSA isolates, indicating the possibility of cross-infection between these isolates. Developing appropriate treatment protocols to prevent the spread of MRSA infections in the community is currently an urgent need.
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Authors
Tahereh Dehghani
Department of Microbiology, Jahrom Branch, Islamic Azad university, Jahrom, Iran.
Afsaneh Karmostaji
Infectious and Tropical Diseases Research Center, Hormozgan Health Institute Hormozgan University of Medical Sciences, Bandar Abbas, Iran.
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