Raman Spectroscopic Characterization of Hepatic Differentiation of Mesenchymal Stem Cells

Background and Aims: Mesenchymal stem cells (MSCs) are a preferred cell source for the generation of hepatocyte-like cells in regenerative medicine. They can be isolated from different sources, including adipose tissues. The Raman spectroscopy approach was evaluated for quick and efficient identification of MSCs differentiation status and a broader perspective on cell differentiation. Materials and Methods: The human adipose-derived mesenchymal stem cells (hASCs) were differentiated toward hepatocyte-like cells using a well-established method. The cells were cultured on fluorescence-free quartz discs, and the efficiency of differentiation was examined using molecular and biochemical methods. The Raman spectra were recorded at days ۱, ۷, ۱۴, and ۲۱ of differentiation, and HepG۲ was used as a positive control. Results: The changes in Raman spectra were detected during the sequential stages of differentiation, and the pattern of peaks on the last day of differentiation was remarkably similar to the positive control (HepG۲). Conclusion: Raman spectroscopy showed considerable potential to characterize hepatic differentiation.

Keywords:

Adipose tissue , Hepatocyte-like cells , Mesenchymal stem cells , Raman spectroscopy