In Vitro Production of Bulblet in Galanthus transcaucasicus Fomin, an Endangered Medicinal Plant
Publish place: Journal of Plant Physiology and Breeding، Vol: 6، Issue: 2
Publish Year: 1395
نوع سند: مقاله ژورنالی
زبان: English
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JR_BREDNG-6-2_001
تاریخ نمایه سازی: 14 اسفند 1401
Abstract:
To optimize conditions for micropropagating Galanthus transcaucasicus Fomin, the effect of explant type, different concentrations of sucrose, type and different concentrations of auxin were examined on bulblet production. For the induction of bulblets, three types of bulb scales were usesd as explants. A two-step sterilization procedure was applied. Assessments were made after incubation for ۲۰ weeks at ۲۳±۱ºC with ۱۶h photoperiods. Highest number of bulblets were proliferated culturing bulb scales on modified MS medium supplemented with ۲.۰ mg.l-۱ BA and ۲.۰ mg.l-۱ IBA. About ۲۷% of bulblets were bigger than ۵ mm in diameter, suitable for acclimatization. With the increase of sucrose concentration, number of bulblets decreased, but their size increased. This protocol provided a basis for future study on large-scale multiplication system for commercial nurseries of Galanthus transcaucasicus.
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Authors
Marzieh Babashpour-Asl
Department of Horticultural Science, Maragheh Branch, Islamic Azad University, Maragheh, Iran
Ali Movafeghi
Department of Plant Biology, Faculty of Natural Sciences, University of Tabriz, Tabriz, Iran
Khadijeh Zare
Department of Biology, Ahar Branch, Islamic Azad University, Ahar, Iran
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