Genetic Variation Among Salvia Species Based on Sequence-Related Amplified Polymorphism (SRAP) Marker

In this study, SRAP molecular maker approach was performed to investigate genetic diversity in the Salvia genus. A total of ۲۰۵ DNA bands were produced from PCR amplification of ۱۱ Salvia species and populations using ۲۵ selective primer combinations, of which ۲۰۴ polymorphic genetic loci accounted. The total number of amplified fragments ranged from ۳ to ۱۵. The genetic similarities of ۱۱ collections were estimated from ۰.۰۹۶۸ to ۰.۶۹۴۹. According to the SRAP marker analysis, S.verticillata۲ and S.nemorosa۲ had the lowest similarityand the species of S.verticillata۲ and S.verticillata۳ had the highest similarity. Unweighted pair-group method with arithmetic averages clustering algorithm categorized Salvia species into six major groups. In general, SRAP markers were able to detect high polymorphism among Salvia genotypes. Hence, the technique is regarded as a reliable tool for differentiation of Salvia at inter and intra-species level.

Keywords:

Cluster analysis , Genetic Diversity , Salvia , SRAP molecular marker