LD۵۰ Acute Toxicity Test of the Anticancer Compound of Dibutyltin (IV) Bis-N-Benzyl Methyl Dithiocarbamate in White Mouse (Mus musculus)

Organotin compounds are organometallic compounds composed of one or more tin-carbon (Sn-C) bonds. Organotin compounds have been shown to have anticancer, antibacterial, and antitumor activities. Based on the several activities of the organotin group, it is hoped that the Dibutyltin (IV) Bis-N-Benzylmethyl Dithiocarbamate compound has the potential to be developed as a new drug candidate. To be a candidate for a new drug, the study was aimed to test and determine the safety level of the compound. Study to determine the LD۵۰ value and category of toxicity of the Dibutyltin (IV) Bis-N-Benzylmethyl Dithiocarbamate compound in white mice (Mus musculus). This research is a laboratory experimental study using ۴۰ white mice, consisting of ۲۰ male mice and ۲۰ female mice. The dose of the test substance was ۲۴۰, ۴۸۰, and ۹۶۰mg/Kg body weight. Observations of mice were carried out for ۲۴ hours by observing the number of dead animals and seeing toxic symptoms, body weight, and ROW (Relative Organ Weight) then the data were analyzed statistically. The results showed that the dose of ۹۶۰ mg caused the most death with LD۵۰ values of ۷۷۶.۲mg and ۷۹۴.۳mg of toxic symptoms, weight loss in mice, and an effect on ROW. This compound can cause death in male and female mice, with the LD۵۰ value of male mice being ۷۷۶.۲mg/kg body weight and female mice at ۷۹۴.۳mg/ kg body weight, and this compound is categorized as slightly toxic.

Keywords:

Dibutyltin (IV) , LD۵۰ , Toxicity Test Anticancer