Evaluation of the emergence of Acinetobacter baumannii isolates containingVIM and SIM isolated from surfaces and equipment of Tehran medicalcenters by PCR

Background and Objective: Excessive and indiscriminate use of antimicrobials in the hospital andcommunity is known as one of the most important factors predisposing to the development andacquisition of new resistance of bacteria to antimicrobials. The aim of this study was to determine thefrequency of drug-resistant vim and sim genes in Acinetobacter baumannii (MDR) isolates isolated fromthe surfaces and equipment of the NICU ward of Tehran Children's Medical Center Hospital using PCR.Methods: In a descriptive cross-sectional study that was conducted over a period of two months, ۱۲۰samples of the desired levels and equipment were collected and sent to the microbiology department of aspecialized laboratory in West Tehran. First, Acinetobacter baumannii was identified and isolated usingculture methods and biochemical tests of strains. Then, to determine the degree of antibiotic resistance,disk diffusion and microdilution broth methods were used according to CLSI guidelines and the meanminimum lethal concentration (MIC) was evaluated. it placed. Finally, the isolates were evaluated for thepresence of vim and sim genes using molecular PCR.Results: The results showed that out of ۱۲۰ samples, (۴۰) (۳۳.۳%) were isolated from Acinetobacterbaumannii that all isolated isolates (۱۰۰%) were sensitive to cholestin. The resistance of the isolatedsamples to the other antibiotics tested was as follows; ۴۰ isolates (۱۰۰%) to imipenem, meropenem andlincomycin, ۳۹ isolates (۹۷.۵%) to ceftazidime, ۳۹ isolates (۹۷.۵%) to ciprofloxacin, ۳۶ isolates (۹۰%) toceftozoxime and oxacillin, ۳۴ isolates (۸۵ %) To gentamicin, ۲۸ isolates (۷۰%) to ampicillin andtetracycline and ۲۷ isolates (۶۷.۵%) to cefotaxime and cefxime. On the other hand, MIC calculation forimipenem and meropenem antibiotics in ۲۴ isolates (۶۰%) was reported at ۶۴ ug ug / ml. Examination ofthe frequency percentage of the studied genes also indicates the presence of vim and sim genes in (۱۷.۵%)(۷ isolates) and (۲.۵%) (۱ isolate) of Acinetobacter baumannii, respectively.Conclusion: Considering the identification of vim and sim genes in a number of isolates of Acinetobacterbaumannides in this study, it is recommended that in addition to preventing the spread of drug-resistantisolates, more extensive studies on phenotypic and genotypic resistance patterns of the bacterium toantimicrobials. To be done in different parts of the world, including Iran.

Keywords:

Acinetobacter baumannii , Beta-lactamase genes , Antibiotic resistance