Upregulation of INSR Gene Expression and Restoration of Melatonin Levels by Tinospora Cordifolia Stem Extract and Alkaloid Rich Fraction against Continuous Light Induced Polycystic Ovary Syndrome in Rodents

Females of childbearing age often suffer from polycystic ovary syndrome (PCOS), a hormonal condition. Symptoms including insulin resistance, high androgen levels, irregular menstrual (estrous) cycles, and polycystic ovaries are linked to this condition. The study's objective is to determine the therapeutic effect of Tinospora cordifolia stemextract and Alkaloid Rich Fraction against continuously Light Induced Polycystic Ovary Syndrome in Sprague Dawley (SD) female rats. Animals were grouped into six groups where the control group received only distilled water and the standard group received Clomiphene citrate ۲۰ mg/Kg. The test groups received Tinospora cordifolia Stem Extract (۲۰۰ mg/kg b.w and ۴۰۰ mg/kg b.w) and Alkaloid Rich Fraction (۱۰ mg/kg b.w and ۲۰ mg/kg b.w) for the period of ۴ weeks after PCOS induction. The results showed a significant reduction in elevated testosterone levels compared to that of the PCOS group (Light Induced Group (L/L ۲۴ hrs Continuous), but follicle-stimulating hormone (FSH), insulin levels reported significant increase compared to that of the L/L PCOS group (Light Induced Group (L/L ۲۴ hrs Continuous) and increased expression of INSR gene and restoration of melatonin levels. From the results, it was concluded that High doses of Tinospora cordifolia Stem Extract (۴۰۰ mg/kg b.w) and Alkaloid Rich Fraction (۲۰ mg/kg b.w) could be effective against Continuously Light Induced Polycystic Ovary Syndrome in Rodents it has due to decreased testosterone levels and increase insulin sensitivity by up-regulating INSR gene expression followed by restoration of melatonin levels. After treatment period a significant decrease in elevated LH levels, testosterone levels, and a significant increase in insulin and follicle-stimulating hormone (FSH) levels when compared to the L/L PCOS group (Light Induced Group (L/L ۲۴ hrs Continuous), as well as increased INSR gene expression and restored melatonin levels.

Keywords:

HPLC , INSR gene , Melatonin , polycystic ovarian syndrome , Tinospora cordifolia