Evaluation of rs۷۹۰۳۱۴۶ Polymorphism in TCF۷L۲ Gene among Type ۲ Diabetes Patients

Background: Diabetes is characterized by decreased insulin sensitivity and is a growing health threat worldwide. Therefore, this study aimed to evaluate the relationship between rs۷۹۰۳۱۴۶ polymorphism in the TCF۷L۲ gene with type ۲ diabetes in East Azerbaijan province, Iran. Materials and method: A total of ۱۰۰ blood samples were collected from diabetic and healthy people. The samples were subjected to specific primers, PCR, and electrophoresis after DNA extraction from all samples and quality control. Finally, the PCR products were treated with RSal restriction enzyme and electrophoresed again, and the target polymorphism was checked. Results: The frequency of genotypes were TT=۳۳.۷%, CC=۱۶.۸% and CT=۴۹.۵% for healthy people and TT=۴۳%, CC=۱۴% and TC=۴۳% in diabetics. The percentage of the T allele in healthy and diabetic people was as much as ۴۲.۱% and ۶۴.۵%, respectively. Conclusion: According to the results, there was probably a relationship between rs۷۹۰۳۱۴۶ polymorphism in the TCF۷L۲ gene and diabetes.

Keywords:

Polymorphism , rs۷۹۰۳۱۴۶ , TCF۷L۲ , Type ۲ diabetes