Amifostine inhibits acrylamide-induced hepatotoxicity by inhibiting oxidative stress and apoptosis
Publish place: Iranian Journal of Basic Medical Sciences، Vol: 26، Issue: 6
Publish Year: 1402
نوع سند: مقاله ژورنالی
زبان: English
View: 155
This Paper With 7 Page And PDF Format Ready To Download
- Certificate
- من نویسنده این مقاله هستم
استخراج به نرم افزارهای پژوهشی:
شناسه ملی سند علمی:
JR_IJBMS-26-6_007
تاریخ نمایه سازی: 21 اردیبهشت 1402
Abstract:
Objective(s): Acrylamide (ACR) is a toxic chemical agent that can induce hepatotoxicity through different mechanisms including oxidative stress and apoptosis. Amifostine is an important hepatoprotective and anti-oxidant compound. In this research, the hepatoprotective effect of amifostine on ACR-induced hepatotoxicity in rats has been investigated.Materials and Methods: Male Wistar rats were randomly divided into ۷ groups, including: ۱. Control group, ۲. ACR (۵۰ mg/kg, ۱۱ days, IP), ۳-۵. ACR+ amifostine (۲۵, ۵۰, ۱۰۰ mg/kg, ۱۱ days, IP), ۶. ACR+ N-acetyl cysteine (NAC) (۲۰۰ mg/kg, ۱۱ days, IP), and ۷. Amifostine (۱۰۰ mg/kg, ۱۱ days, IP). At the end of the injection period, animals’ liver samples were collected to determine the content of glutathione (GSH), malondialdehyde (MDA), and apoptotic proteins (B-cell lymphoma ۲ (Bcl۲), Bcl-۲-associated X protein (Bax), and cleaved caspase-۳. Serum samples were also collected to measure alanine transaminase (ALT) and aspartate transaminase (AST) levels. Results: Administration of ACR increased MDA, Bax/Bcl۲ ratio, cleaved caspase-۳, ALT, and AST levels, and decreased GSH content compared with the control group. The administration of amifostine with ACR decreased MDA, Bax/Bcl۲ ratio, cleaved caspase-۳, ALT, and AST levels, and increased GSH content compared with the ACR group. Receiving NAC along with ACR reversed the alterations induced by ACR. Conclusion: This study shows that pretreatment with amifostine can reduce ACR-induced toxicity in the liver tissue of rats. Since oxidative stress is one of the most important mechanisms in ACR toxicity, amifostine probably reduces the toxicity of ACR by increasing the anti-oxidant and anti-apoptotic capacity of the hepatic cells.
Keywords:
Authors
Mostafa Karimi
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Mahboobeh Ghasemzadeh Rahbardar
Pharmaceutical Research Center, Pharmaceutical Technology Institute, Mashhad University of Medical Sciences, Mashhad, Iran
Bibi Marjan Razavi
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
Hossein Hosseinzadeh
Department of Pharmacodynamics and Toxicology, School of Pharmacy, Mashhad University of Medical Sciences, Mashhad, Iran
مراجع و منابع این Paper:
لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :