Evaluation of the Immunogenicity of Recombinant Espb, Espc Proteins from Mycobacterium Tuberculosis and the Fusion Espc/Espb Protein in BALB/C Mice

Publish Year: 1401
نوع سند: مقاله ژورنالی
زبان: English
View: 122

This Paper With 9 Page And PDF Format Ready To Download

  • Certificate
  • من نویسنده این مقاله هستم

استخراج به نرم افزارهای پژوهشی:

لینک ثابت به این Paper:

شناسه ملی سند علمی:

JR_RBMB-11-4_006

تاریخ نمایه سازی: 6 خرداد 1402

Abstract:

Background: Two newly identified proteins, EspB and EspC are involved in the pathogenesis of Mycobacterium tuberculosis. The objective of the present study was to evaluate the immunogenicity of recombinant EspC, EspB, and EspC/EspB fusion proteins in mice. Methods: BALB/c mice were immunized subcutaneously with recombinant EspC, EspB, and fusion EspC/EspB proteins, three times with along with Quil-A as an adjuvant. The cellular and humoral immune responses were evaluated by quantifying IFN-g, IL-۴, IgG, IgG۱, and IgG۲a antibodies against the antigens. Results: The results showed that the mice immunized with recombinant EspC, EspB, and EspC/EspB proteins did not produce IL-۴, whereas IFN-g was secreted in response to all three proteins. EspC/EspB group produced significant amounts of IFN-g in response to stimulation with all the three recombinant proteins (P<۰.۰۰۱). In mice immunized with EspC, high levels of IFN-g were detected in response to EspC/EspB, and EspC (P<۰.۰۰۰۱); while mice immunized with EspB produced lower levels of IFN- g in response to EspC/EspB, and EspB (P<۰.۰۵). Conclusions: All the three recombinant proteins induced Th۱-type immune responses in mice against EspB and EspC; however, EspC/EspB protein is more desirable due to the presence of epitopes from both EspC and EspB proteins and the production of immune responses against both.

Authors

Omid Salemi

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Zahra Noormohammadi

Department of Biology, Science and Research Branch, Islamic Azad University, Tehran, Iran.

Fariborz Bahrami

Department of Immunology, Pasteur Institute of Iran, ۶۹ Pasteur Ave, Tehran ۱۳۱۶۹-۴۳۵۵۱, Iran.

Seyed Davar Siadat

Department of Mycobacteriology and pulmonary research, Pasteur Institute of Iran, ۶۹ Pasteur Ave., Tehran ۱۳۱۶۹-۴۳۵۵۱, Iran.

Soheila Ajdary

Department of Immunology, Pasteur Institute of Iran, ۶۹ Pasteur Ave, Tehran ۱۳۱۶۹-۴۳۵۵۱, Iran.

مراجع و منابع این Paper:

لیست زیر مراجع و منابع استفاده شده در این Paper را نمایش می دهد. این مراجع به صورت کاملا ماشینی و بر اساس هوش مصنوعی استخراج شده اند و لذا ممکن است دارای اشکالاتی باشند که به مرور زمان دقت استخراج این محتوا افزایش می یابد. مراجعی که مقالات مربوط به آنها در سیویلیکا نمایه شده و پیدا شده اند، به خود Paper لینک شده اند :
  • Bloom BR, Atun R. Back to the future: Rethinking global ...
  • Houben RM, Dodd PJ. The Global Burden of Latent Tuberculosis ...
  • Furin J, Cox H, Pai M. Tuberculosis. Lancet. ۲۰۱۹;۳۹۳(۱۰۱۸۱):۱۶۴۲-۵۶ ...
  • Abubakar I, Pimpin L, Ariti C, Beynon R, Mangtani P, ...
  • Finlay BB, Falkow S. Common themes in microbial pathogenicity revisited. ...
  • https://doi.org/۱۰.۱۱۲۸/mmbr.۶۱.۲.۱۳۶-۱۶۹.۱۹۹۷Simeone R, Bottai D, Frigui W, Majlessi L, Brosch R. ...
  • Abdallah AM, Gey van Pittius NC, Champion PA, Cox J, ...
  • Clemmensen HS, Knudsen NPH, Rasmussen EM, Winkler J, Rosenkrands I, ...
  • Wong KW. The Role of ESX-۱ in Mycobacterium tuberculosis Pathogenesis. ...
  • Solomonson M, Setiaputra D, Makepeace KAT, Lameignere E, Petrotchenko EV, ...
  • Xu J, Laine O, Masciocchi M, Manoranjan J, Smith J, ...
  • Satchidanandam V, Amara RR, Uchil PD, Singh V. The regulatory ...
  • Fortune SM, Jaeger A, Sarracino DA, Chase MR, Sassetti CM, ...
  • Millington KA, Fortune SM, Low J, Garces A, Hingley-Wilson SM, ...
  • McLaughlin B, Chon JS, MacGurn JA, Carlsson F, Cheng TL, ...
  • Sidders B, Pirson C, Hogarth PJ, Hewinson RG, Stoker NG, ...
  • Salemi O, Noormohammadi Z, Bahrami F, Siadat SD, Ajdary S. ...
  • Martin C. Tuberculosis vaccines: past, present and future. Curr Opin ...
  • Barker LF, Brennan MJ, Rosenstein PK, Sadoff JC. Tuberculosis vaccine ...
  • van Dissel JT, Arend SM, Prins C, Bang P, Tingskov ...
  • Gao LY, Guo S, McLaughlin B, Morisaki H, Engel JN, ...
  • Gijsbers A, Vinciauskaite V, Siroy A, Gao Y, Tria G, ...
  • Chen JM, Zhang M, Rybniker J, Boy-Rottger S, Dhar N, ...
  • Lou Y, Rybniker J, Sala C, Cole ST. EspC forms ...
  • Zeng G, Zhang G, Chen X. Th۱ cytokines, true functional ...
  • Domingo-Gonzalez R, Prince O, Cooper A, Khader SA. Cytokines and ...
  • Xu G, Wang J, Gao GF, Liu CH. Insights into ...
  • da Silva MV, Tiburcio MG, Machado JR, Silva DA, Rodrigues ...
  • Tan S, Lin N, Huang M, Wang Q, Tan Y, ...
  • Zhang X, Su Z, Zhang X, Hu C, Yu J, ...
  • Kong H, Dong C, Xiong S. A novel vaccine p۸۴۶ ...
  • نمایش کامل مراجع