Research Article: Blood and enzyme factors changes in New Zealand rabbit (Oryctolagus cuniculus) as an animal model in response to killed hemorrhagic septicemia virus (VHSv) virulent to Oncorhynchus mykiss

The objectives to this research were to determine the haematological changes in Oryctolagus cuniculus as an animal model in response to killed hemorrhagic septicemia virus (VHSV) virulent to Oncorhynchus mykiss. This study composed of ۵ groups; Treatment ۱ (T۱) with ۱cc virus, treatment ۲ (T۲) with ۰.۵cc virus and ۰.۵cc adjuvant, treatment ۳ (T۳) with ۰.۷۵cc virus and ۰.۲۵cc adjuvant, treatment ۴ (T۴) with ۰.۲۵cc virus and ۰.۷۵cc adjuvant, and a control for a period of ۴ months, once a month IM injection in ۵ group of three New Zealand rabbits with health certification prepared by Razi Vaccine and Serum Research Institute. After ۱۵ days, blood parameters, hematocrit (HCT), hemoglobin (HGB), mean hemoglobin concentration in red blood cells (MCH), red blood cells (RBC), average concentration of red blood cell (MCHC), white blood cells (WBC), neutrophils (SEG), lymphocytes (Lyn), eosinophil (Eos), aspirate transaminase (AST) and alanine transaminase (ALT).The results  showed that ALT, AST, HCT, HGB and WBC concentrations of all rabbits significantly were difference (p < ۰.۰۵). T۲ (۰.۵cc virus and ۰.۵cc adjuvant) and T۳ (۰.۷۵cc virus and ۰.۲۵cc adjuvant) showed higher ALT, AST, HCT and WBCs among other treatments in all ۴ blood samplings and among these treatments, T۲ with lower levels of ALT and AST (indicating less pressure on the liver) and higher levels of WBCs which can lead to greater amount of neutralizing polyclonal antibodies indicated better results.

Keywords:

Viral Hemorrhagic Septicemia virus , Rabbit , Hematology , Enzymes