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An Overview of Culture Conditions for Recombinant Protein Expression in Escherichia coli

Publish place: Journal of Applied Biotechnology Reports، Vol: 10، Issue: 1

Publish Year: 1402

Type: Journal paper

Language: English

View: 169

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https://civilica.com/doc/1683272

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JR_JABR-10-1_001

Index date: 27 June 2023

An Overview of Culture Conditions for Recombinant Protein Expression in Escherichia coli abstract

Escherichia coli is one of the most commonly used organisms for producing recombinant protein. The recognized cell genome and well-known cell factory of the bacteria make it an ideal heterologous system of preference for the production of recombinant proteins. Over recent years, this cell system has been modified to improve the production of therapeutic proteins, and several methodologies are now available. One of the scientists' most preferred strategies is maintaining cell survival by optimizing the culture conditions. This review summarises some experiments related to those culture conditions and discusses how they affect recombinant protein expression in E. coli.

An Overview of Culture Conditions for Recombinant Protein Expression in Escherichia coli Keywords:

Recombinant protein , Escherichia coli , Culture Expression Parameters

An Overview of Culture Conditions for Recombinant Protein Expression in Escherichia coli authors

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, ۴۵۳۶۳, Indonesia

Firdausi Permata Latifah

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, ۴۵۳۶۳, Indonesia

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, ۴۵۳۶۳, Indonesia

Sriwidodo Sriwidodo

Department of Pharmacy and Pharmaceutical Technology, Faculty of Pharmacy, Universitas Padjadjaran, Sumedang, ۴۵۳۶۳, Indonesia

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, ۴۵۳۶۳, Indonesia

Mamlikatu Azizah

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, ۴۵۳۶۳, Indonesia

Department of Chemistry, Faculty of Mathematics and Natural Sciences, Universitas Padjadjaran, Sumedang, ۴۵۳۶۳, Indonesia

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I'm the author of the paper

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Link to this Paper:

https://civilica.com/doc/1683272

Document National Code:

JR_JABR-10-1_001

Index date: 27 June 2023

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Maksum, Iman and Latifah, Firdausi Permata and Nabiel, Ahmad and Sriwidodo, Sriwidodo and Lestari, Niky and Azizah, Mamlikatu and Utami, Diandra,1402,An Overview of Culture Conditions for Recombinant Protein Expression in Escherichia coli,https://civilica.com/doc/1683272

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